Comoglio P M, Guglielmone R
Immunology. 1973 Jul;25(1):71-80.
The serum of mice with IgA-secreting transplantable myelomas contains high IgA concentrations, and polymeric IgA with secretory characteristics are observed in the intestinal and salivary secretions. Immunofluorescence was used to demonstrate the transepithelial transfer of these secretory IgAs. IgA molecules were noted within the cytoplasm of intestinal columnar and salivary-secreting cells; they were not present in goblet cells or the salivary-excretory duct cells. Purified IgAs from the intestinal secretions of myeloma-bearing mice possess extra antigenic determinants attributable to an additional secretory piece. A specific antisecretory piece antiserum did not stain either the intestinal columnar cells or the salivary secreting cells, whereas goblet cells and salivary duct cells were shown to contain this piece. Immunofluorescence also showed that IgA and the secretory piece were simultaneously present in the luminal surface of the intestinal epithelium.
患有可移植性分泌 IgA 的骨髓瘤的小鼠血清中 IgA 浓度很高，并且在肠道和唾液分泌物中观察到具有分泌特性的聚合 IgA。利用免疫荧光来证明这些分泌型 IgA 的跨上皮转运。在肠柱状细胞和唾液分泌细胞的细胞质内发现了 IgA 分子；它们不存在于杯状细胞或唾液排泄管细胞中。从患有骨髓瘤的小鼠肠道分泌物中纯化的 IgA 具有额外的抗原决定簇，这归因于额外的分泌片。一种特异性抗分泌片抗血清既不使肠柱状细胞也不使唾液分泌细胞染色，而杯状细胞和唾液导管细胞显示含有这种分泌片。免疫荧光还表明，IgA 和分泌片同时存在于肠上皮的腔面。
