Carruthers M M, Anderson B
J Infect Dis. 1979 Jul;140(1):119-22. doi: 10.1093/infdis/140.1.119.
Rapid adherence of Kanagawa-positive Vibrio parahaemolyticus to human fetal intestinal cells has been demonstrated in monolayer cultures. In this study, polyanionic carbohydrates and glycoproteins of various biochemical compositions, structural configurations, and molecular weights were found to inhibit this adherence. Mono- and disaccharide components of inhibitory compounds did not themselves inhibit adherence. Levels of Ca++ in the test mixture were similar in both the presence and the absence of chondroitin sulfate, a potent inhibitor of adherence by V. parahaemolyticus. These results, which demonstrate a major effect of surface charge in this model of bacterial adherence, suggest that differences in surface charge may contribute to the varying degrees of adherence by different strains of V. parahaemolyticus. This striking effect of surface charge on adherence by V. parahaemolyticus underscores the importance of experimental conditions and of substances that affect the surface potential of the cell in the interpretation of findings in models of bacterial adherence.
在单层培养中已证实，神奈川阳性副溶血性弧菌能迅速黏附于人类胎儿肠道细胞。在本研究中，发现各种生化组成、结构构型和分子量的聚阴离子碳水化合物和糖蛋白可抑制这种黏附。抑制性化合物的单糖和双糖成分本身并不抑制黏附。在存在和不存在硫酸软骨素（一种副溶血性弧菌黏附的有效抑制剂）的情况下，测试混合物中的钙离子水平相似。这些结果表明表面电荷在这种细菌黏附模型中具有主要作用，提示表面电荷的差异可能导致不同菌株的副溶血性弧菌黏附程度不同。表面电荷对副溶血性弧菌黏附的这种显著影响突出了实验条件以及影响细胞表面电位的物质在解释细菌黏附模型研究结果中的重要性。
