Merrell B R, Walker R I, Joseph S W
Can J Microbiol. 1984 Mar;30(3):381-8. doi: 10.1139/m84-056.
The initial interaction and adherence of Vibrio parahemolyticus to epithelial tissue culture cells, human buccal epithelial cells, and the ileal mucosa of mice were studied. Using scanning electron microscopy, adherent bacteria were observed only on degenerating human embryonic intestinal, HeLa, and buccal cells; healthy normal cells were devoid of bacteria. Sheared V. parahaemolyticus, i.e., lacking flagella, did not adhere to either normal or degenerating tissue cells. Neither ultraviolet-inactivated organisms nor cell-free culture supernate affected the epithelial cells. Similar findings were observed on the mucosa of the ileum in mice inoculated with V. parahaemolyticus. It appears that V. parahaemolyticus possesses a cytotoxic factor which alters epithelial cells. This factor appears to be closely associated with viable organisms and may be a functional element in the adherence process of flagellated V. parahaemolyticus to mammalian epithelial cells.
研究了副溶血性弧菌与上皮组织培养细胞、人颊上皮细胞以及小鼠回肠黏膜的初始相互作用和黏附情况。通过扫描电子显微镜观察到，仅在退化的人胚胎肠道、HeLa细胞和颊细胞上发现了黏附的细菌；健康的正常细胞上没有细菌。剪切后的副溶血性弧菌，即缺乏鞭毛的细菌，不黏附于正常或退化的组织细胞。紫外线灭活的生物体或无细胞培养上清液均未影响上皮细胞。在用副溶血性弧菌接种的小鼠回肠黏膜上也观察到了类似的结果。似乎副溶血性弧菌具有一种可改变上皮细胞的细胞毒性因子。该因子似乎与活生物体密切相关，可能是有鞭毛的副溶血性弧菌黏附于哺乳动物上皮细胞过程中的一个功能元件。
