一种检测低剂量紫外线照射细菌DNA中嘧啶二聚体的方法。
A method for detecting pyrimidine dimers in the DNA of bacteria irradiated with low doses of ultraviolet light.
作者信息
Ganesan A K
出版信息
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2753-6. doi: 10.1073/pnas.70.10.2753.
Abstract
Cells of Escherichia coli treated with lysozyme and Brij-58 become permeable to proteins, but do not release their DNA. I incubated permeable cells with an endonuclease that produces single-strand breaks in DNA-containing pyrimidine dimers. The enzyme entered the permeable cells, and, if they had been irradiated with ultraviolet light, caused breaks in their DNA. The frequency of breaks was estimated from the sedimentation pattern of the DNA in alkaline sucrose gradients. The procedure is sensitive enough to detect the dimers produced by a dose of 10 erg/mm(2) at 254 nm, or about 50 dimers per E. coli genome. This method exemplifies and extends the use of permeabilized cells for examining biological processes at the molecular level.
摘要
用溶菌酶和Brij - 58处理的大肠杆菌细胞对蛋白质具有通透性，但不会释放其DNA。我将通透细胞与一种能在含嘧啶二聚体的DNA中产生单链断裂的核酸内切酶一起孵育。该酶进入通透细胞，并且，如果这些细胞已用紫外线照射过，就会导致其DNA断裂。通过碱性蔗糖梯度中DNA的沉降模式来估计断裂频率。该方法灵敏度足以检测在254nm下10尔格/平方毫米剂量产生的二聚体，或大约每大肠杆菌基因组50个二聚体。这种方法例证并扩展了利用通透细胞在分子水平上研究生物学过程的应用。
相似文献
1
A method for detecting pyrimidine dimers in the DNA of bacteria irradiated with low doses of ultraviolet light.一种检测低剂量紫外线照射细菌DNA中嘧啶二聚体的方法。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2753-6. doi: 10.1073/pnas.70.10.2753.
2
Persistence of pyrimidine dimers during post-replication repair in ultraviolet light-irradiated Escherichia coli K12.紫外线照射的大肠杆菌K12在复制后修复过程中嘧啶二聚体的持续存在。
J Mol Biol. 1974 Jul 25;87(1):103-19. doi: 10.1016/0022-2836(74)90563-4.
3
An enzymatic assay for pyrimidine dimers in DNA.
Basic Life Sci. 1975;5A:67-9. doi: 10.1007/978-1-4684-2895-7_10.
4
Measurement of thymine dimers in DNA by thin-layer chromatography.
Anal Biochem. 1973 May;53(1):124-31. doi: 10.1016/0003-2697(73)90413-2.
5
Mechanism of post-irradiation degradation of deoxyribonucleic acid in a radiosensitive Escherichia coli (NG30) irradiated with ultraviolet light.
J Mol Biol. 1972 Mar 28;65(2):213-25. doi: 10.1016/0022-2836(72)90278-1.
6
T4-endonuclease V-sensitive sites in DNA from ultraviolet-irradiated human cells.紫外线照射的人类细胞DNA中对T4核酸内切酶V敏感的位点。
Biochim Biophys Acta. 1976 Apr 2;425(4):428-37. doi: 10.1016/0005-2787(76)90007-1.
7
Endonuclease-sensitive sites in the DNA of irradiated bacteria: a rapid and sensitive assay.受辐照细菌DNA中的核酸内切酶敏感位点：一种快速灵敏的检测方法。
Biochim Biophys Acta. 1973 Jun 8;312(1):33-7. doi: 10.1016/0005-2787(73)90049-x.
8
T4 endonuclease involved in repair of DNA.参与DNA修复的T4核酸内切酶。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839-45. doi: 10.1073/pnas.67.4.1839.
9
[Repair mechanisms and mutagenesis in Escherichia coli bacteria irradiated with ultraviolet light].[紫外线照射的大肠杆菌中的修复机制与诱变]
Usp Sovrem Biol. 1978 May-Jun;85(3):340-59.
10
Biochemical studies on radiation-sensitive mutations in bacteriophage T4-1.噬菌体T4-1辐射敏感突变的生化研究。
J Biochem. 1975 Feb;77(2):303-11. doi: 10.1093/oxfordjournals.jbchem.a130727.
引用本文的文献
1
Photobiological Origins of the Field of Genomic Maintenance.基因组维持领域的光生物学起源。
Photochem Photobiol. 2016 Jan-Feb;92(1):52-60. doi: 10.1111/php.12542. Epub 2015 Dec 16.
2
Accumulation of nuclear DNA damage or neuron loss: molecular basis for a new approach to understanding selective neuronal vulnerability in neurodegenerative diseases.核DNA损伤的积累或神经元丢失：理解神经退行性疾病中选择性神经元易损性新方法的分子基础。
DNA Repair (Amst). 2008 Jul 1;7(7):1087-97. doi: 10.1016/j.dnarep.2008.03.010. Epub 2008 May 23.
3
Excision of pyrimidine dimers from nuclear deoxyribonucleic acid in ultraviolet-irradiated Dictyostelium discoideum.紫外线照射的盘基网柄菌中核脱氧核糖核酸嘧啶二聚体的切除
Mol Cell Biol. 1981 Feb;1(2):121-7. doi: 10.1128/mcb.1.2.121-127.1981.
4
Postreplication repair of ultraviolet-irradiated transforming deoxyribonucleic acid in Bacillus subtilis.枯草芽孢杆菌中紫外线照射的转化脱氧核糖核酸的复制后修复
J Bacteriol. 1981 Jan;145(1):434-41. doi: 10.1128/jb.145.1.434-441.1981.
5
Postreplication repair of deoxyribonucleic acid and daughter strand exchange in uvr- mutants of Bacillus subtilis.枯草芽孢杆菌uvr-突变体中脱氧核糖核酸的复制后修复及子链交换
J Bacteriol. 1980 Nov;144(2):840-3. doi: 10.1128/jb.144.2.840-843.1980.
6
UV irradiation impairs in vivo encapsidation of bacteriophage T4 DNA.紫外线照射会损害噬菌体T4 DNA在体内的包装。
J Virol. 1984 May;50(2):293-300. doi: 10.1128/JVI.50.2.293-300.1984.
7
Ultraviolet irradiation inhibits encapsidation of simian virus 40 chromatin.紫外线照射会抑制猴病毒40染色质的衣壳化。
J Virol. 1981 Dec;40(3):729-34. doi: 10.1128/JVI.40.3.729-734.1981.
8
Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli.克隆于大肠杆菌中的噬菌体T4的denV基因的表达。
Proc Natl Acad Sci U S A. 1981 May;78(5):2796-800. doi: 10.1073/pnas.78.5.2796.
9
Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA.嘧啶二聚体DNA糖基化酶与脱嘌呤/脱嘧啶DNA内切核酸酶的物理关联对紫外线损伤DNA的修复至关重要。
Proc Natl Acad Sci U S A. 1981 May;78(5):2742-6. doi: 10.1073/pnas.78.5.2742.
10
Detection of pyrimidine dimers in hydrolysates of yeast DNA by high voltage paper electrophoresis.
Mol Gen Genet. 1974 Jun 27;130(4):291-6. doi: 10.1007/BF00333867.
本文引用的文献
1
THYMINE DIMERS AND INHIBITION OF DNA SYNTHESIS BY ULTRAVIOLET IRRADIATION OF CELLS.胸腺嘧啶二聚体与紫外线照射细胞对DNA合成的抑制作用
Science. 1963 Dec 13;142(3598):1464-6. doi: 10.1126/science.142.3598.1464.
2
THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION.氨基酸剥夺对后续脱氧核糖核酸复制的影响。
Biochim Biophys Acta. 1963 Sep 17;76:9-24.
3
Thymine deficiency and the normal DNA replication cycle. I.胸腺嘧啶缺乏与正常DNA复制周期。I.
J Mol Biol. 1961 Apr;3:144-55. doi: 10.1016/s0022-2836(61)80041-7.
4
Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.受辐照大肠杆菌脱氧核糖核酸的体内重建；断裂片段的重新连接。
Nature. 1966 Oct 29;212(5061):534-5. doi: 10.1038/212534a0.
5
T4 endonuclease involved in repair of DNA.参与DNA修复的T4核酸内切酶。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839-45. doi: 10.1073/pnas.67.4.1839.
6
Adenosine triphosphate-dependent synthesis of biologically active DNA by azide-poisoned bacteria.叠氮化物中毒细菌对生物活性DNA的三磷酸腺苷依赖性合成。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1296-300. doi: 10.1073/pnas.68.6.1296.
7
Lysis of Escherichia coli with a neutral detergent.用中性去污剂裂解大肠杆菌。
Biochim Biophys Acta. 1967 Dec 19;149(2):476-88. doi: 10.1016/0005-2787(67)90175-x.
8
Repair of DNA studied with a nuclease specific for UV-induced lesions.用对紫外线诱导损伤具有特异性的核酸酶研究DNA修复。
Proc Natl Acad Sci U S A. 1966 Sep;56(3):932-9. doi: 10.1073/pnas.56.3.932.
9
Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.噬菌体T4对紫外线照射的脱氧核糖核酸的暗修复：一种二聚体特异性噬菌体诱导的内切核酸酶的纯化与特性分析
J Bacteriol. 1971 May;106(2):500-7. doi: 10.1128/jb.106.2.500-507.1971.
10
Correlations between host-cell reactivation, ultraviolet reactivation and pyrimidine dimer excision in the DNA of bacteriophage lambda.噬菌体λ DNA 中宿主细胞复活、紫外线复活与嘧啶二聚体切除之间的相关性
J Mol Biol. 1970 Jul 14;51(1):131-44. doi: 10.1016/0022-2836(70)90275-5.
Zotero 插件