大肠杆菌中多肽链起始的一种特异性抑制剂。
A specific inhibitor of polypeptide-chain initiation in Escherichia coli.
作者信息
Lee-Huang S, Lee H, Ochoa S
出版信息
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2874-8. doi: 10.1073/pnas.70.10.2874.
An inhibitor of polypeptide-chain initiation was isolated from E. coli cells. This protein inhibits formation of the 30S or 70S initiation complex with either fMet-tRNA(f) as initiator and AUG, MS2 RNA, or late T4 RNA as messenger, or acPhe-tRNA as initiator and poly(U) as messenger. Chain elongation, e.g., poly(U) translation at high Mg(2+) concentration, is not inhibited. The inhibitor is rendered ineffective when active aminoacylation of tRNA is taking place, e.g., during natural mRNA translation. This inhibitor is distinct from the so-called interference (i) factors, which interfere exclusively with the action of initiation factor 3. Since the new inhibitor can apparently be turned on and off, it may have a regulatory function in translation.
从大肠杆菌细胞中分离出一种多肽链起始抑制剂。这种蛋白质抑制30S或70S起始复合物的形成，该复合物以fMet-tRNA(f)作为起始物且以AUG、MS2 RNA或晚期T4 RNA作为信使，或者以acPhe-tRNA作为起始物且以聚尿苷酸（poly(U)）作为信使。链延伸，例如在高镁离子（Mg(2+)）浓度下的聚尿苷酸（poly(U)）翻译，不受抑制。当tRNA进行活性氨酰化时，例如在天然mRNA翻译过程中，该抑制剂变得无效。这种抑制剂不同于所谓的干扰（i）因子，干扰（i）因子仅干扰起始因子3的作用。由于这种新抑制剂显然可以开启和关闭，它可能在翻译中具有调节功能。
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