Hermoso J M, Szer W
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4708-12. doi: 10.1073/pnas.71.12.4708.
The MS2 RNA binding capacity of 30S ribosomal subunits, which is lost when protein S1 is removed, can be restored following incubation with interference factor ialpha and repelleting. Polyacrylamide-agarose gel electrophoresis shows that, under these conditions, a faster moving, non-RNA binding 30S species, which contains no S1, is converted to a slower moving RNA-binding one, having the same mobility as the 30S species that contains protein S1. Factor ialpha binds to single-stranded RNAs in a pattern that closely resembles the RNA binding pattern of initiation factor IF-3.
30S核糖体亚基的MS2 RNA结合能力在去除蛋白质S1时丧失，在与干扰因子α孵育并重新沉淀后可恢复。聚丙烯酰胺-琼脂糖凝胶电泳显示，在这些条件下，一种不含S1、迁移速度较快的非RNA结合30S物种转变为迁移速度较慢的RNA结合物种，其迁移率与含有蛋白质S1的30S物种相同。因子α以一种与起始因子IF-3的RNA结合模式非常相似的方式与单链RNA结合。
