通过T细胞膜标志物的免疫荧光法证实裸鼠中T细胞前体的分化。
Differentiation of T-cell precursors in nude mice demonstrated by immunofluorescence of T-cell membrane markers.
作者信息
Loor F, Kindred B
出版信息
J Exp Med. 1973 Nov 1;138(5):1044-55. doi: 10.1084/jem.138.5.1044.
Abstract
When the thymus from an AKR mouse (TL(-), theta-AKR) is grafted to a BALB/c-nu/nu mouse (TL2, theta-C3H), the grafted thymus is rapidly repopulated by host lymphocytes, i.e., lymphocytes having the TL2 and theta-C3H T-lymphocyte membrane antigen markers. theta-C3H lymphocytes also appear rapidly in the spleen and lymph nodes. After a few weeks, BALB/c nude mice grafted with AKR thymus and normal BALB/c mice could not be distinguished on the basis of the number of TL-positive thymocytes or theta-C3H-positive lymphocytes in thymus, spleen, or lymph nodes. These experiments give a definitive proof of the existence of precursor cells for the T compartment of the lymphoid system in the nude mouse. They strongly suggest the involvement of host-derived T cells in the recovery of some T-cell functions by nude mice grafted with allogeneic thymuses.
摘要
当将AKR小鼠（TL(-)，θ-AKR）的胸腺移植到BALB/c-nu/nu小鼠（TL2，θ-C3H）时，移植的胸腺会迅速被宿主淋巴细胞重新填充，即具有TL2和θ-C3H T淋巴细胞膜抗原标记的淋巴细胞。θ-C3H淋巴细胞也会迅速出现在脾脏和淋巴结中。几周后，移植了AKR胸腺的BALB/c裸鼠和正常BALB/c小鼠在胸腺、脾脏或淋巴结中TL阳性胸腺细胞或θ-C3H阳性淋巴细胞的数量上无法区分。这些实验明确证明了裸鼠淋巴系统T区室前体细胞的存在。它们强烈表明宿主来源的T细胞参与了移植同种异体胸腺的裸鼠某些T细胞功能的恢复。
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