Suppr超能文献

用于估计抗体量和亲和力的硫酸铵沉淀技术的有效性。

The validity of the ammonium sulphate precipitation technique of estimation of antibody amount and avidity.

作者信息

Ahlstedt S, Holmgren J, Hanson L A

出版信息

Immunology. 1973 Dec;25(6):917-22.

PMID:4586286
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1422995/
Abstract

Determinations of antibody titres with the ammonium sulphate precipitation technique showed an accuracy of ± 10 per cent (<0.05). Calculation of antibody avidities with Sips' distribution formula, using suitably diluted antisera, gave a ten times greater accuracy than either undiluted or too highly diluted antisera. The accuracy was lower with the formula of Celada, Schmidt and Strom (1969) probably due to the narrow antigen ranges employed. Comparison of avidity calculation according to Sips (1948) and Celada . (1969) showed a good correlation ( = 0.73–0.79; <0.01). No correlation between antibody titre and antibody avidity values in the sera ( = 0.03–0.19) was observed.

摘要

采用硫酸铵沉淀技术测定抗体效价，其准确度为±10%（<0.05）。使用适当稀释的抗血清，根据西普斯分布公式计算抗体亲和力，其准确度比未稀释或过度稀释的抗血清高出十倍。使用塞拉达、施密特和施特罗姆（1969年）公式计算时，准确度较低，这可能是由于所采用的抗原范围较窄。根据西普斯（1948年）和塞拉达（1969年）方法计算亲和力的比较显示出良好的相关性（r = 0.73 - 0.79；<0.01）。未观察到血清中抗体效价与抗体亲和力值之间存在相关性（r = 0.03 - 0.19）。

相似文献

1
The validity of the ammonium sulphate precipitation technique of estimation of antibody amount and avidity.用于估计抗体量和亲和力的硫酸铵沉淀技术的有效性。
Immunology. 1973 Dec;25(6):917-22.
2
Protective capacity of antibodies against E. coli O antigen with special reference to the avidity.抗大肠杆菌O抗原抗体的保护能力，特别提及亲和力。
Int Arch Allergy Appl Immunol. 1974;46(3):470-80. doi: 10.1159/000231150.
3
Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class.实验性肾盂肾炎中的局部抗体产生：数量、亲和力及免疫球蛋白类别
Infect Immun. 1974 Sep;10(3):411-5. doi: 10.1128/iai.10.3.411-415.1974.
4
Experimental Escherichia coli O6 pyelonephritis in rabbits. Effect on O6 antibody quantity and avidity of prior immunization with E. coli O2 bacteria.家兔实验性大肠杆菌O6肾盂肾炎。大肠杆菌O2细菌预先免疫对O6抗体数量和亲和力的影响。
Immunology. 1975 May;28(5):841-6.
5
Antibodies against Escherichia coli O antigen. Antibody amounts and avidities measured with ammonium sulfate precipitation technique.
Int Arch Allergy Appl Immunol. 1976;50(2):164-71.
6
Amount and avidity of antibody to Escherichia coli O antigen measured with the ammonium sulphate precipitation technique in children with urinary tract infections.
Int Arch Allergy Appl Immunol. 1975;49(5):615-26. doi: 10.1159/000231443.
7
Complement activation in relation to protective function of antibodies to Escherichia coli O antigens.补体激活与抗大肠杆菌O抗原抗体的保护功能的关系
Scand J Immunol. 1975 Sep;4(5-6):511-5. doi: 10.1111/j.1365-3083.1975.tb02656.x.
8
Mechanisms of endotoxin tolerance. 8. Specificity of serum transfer.内毒素耐受的机制。8. 血清转移的特异性。
J Immunol. 1973 Nov;111(5):1349-60.
9
Significance of amount and avidity of E. coli O antibodies for manifestation of their serological and protective properties. A preliminary study.大肠杆菌O抗体的数量和亲和力对其血清学及保护特性表现的意义。一项初步研究。
Int Arch Allergy Appl Immunol. 1972;42(6):826-35. doi: 10.1159/000230661.
10
Determination of soluble immune complex molar composition and antibody association constants by ammonium sulfate precipitation.通过硫酸铵沉淀法测定可溶性免疫复合物的摩尔组成和抗体结合常数。
J Immunol. 1974 Feb;112(2):451-61.

引用本文的文献

1
Preparation of mouse anti-human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection.小鼠抗人轮状病毒VP7单克隆抗体的制备及其对轮状病毒感染的保护作用
Exp Ther Med. 2019 Aug;18(2):1384-1390. doi: 10.3892/etm.2019.7708. Epub 2019 Jun 25.
2
Antibody-dependent cell-mediated cytotoxicity against Escherichia coli O antigens.针对大肠杆菌O抗原的抗体依赖性细胞介导的细胞毒性
Eur J Clin Microbiol. 1982 Feb;1(1):59-65. doi: 10.1007/BF02014142.
3
Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class.实验性肾盂肾炎中的局部抗体产生：数量、亲和力及免疫球蛋白类别
Infect Immun. 1974 Sep;10(3):411-5. doi: 10.1128/iai.10.3.411-415.1974.
4
Experimental Escherichia coli O6 pyelonephritis in rabbits. Effect on O6 antibody quantity and avidity of prior immunization with E. coli O2 bacteria.家兔实验性大肠杆菌O6肾盂肾炎。大肠杆菌O2细菌预先免疫对O6抗体数量和亲和力的影响。
Immunology. 1975 May;28(5):841-6.
5
A proposition on the distribution of antibody affinities, with implications for the mechanism of B-cell activation.一项关于抗体亲和力分布的命题，对B细胞激活机制具有启示意义。
Immunology. 1975 Dec;29(6):989-99.
6
Immunosuppression in murine malaria. II. The primary response to bovine serum albumin.鼠疟中的免疫抑制。II. 对牛血清白蛋白的初次反应。
Immunology. 1977 Aug;33(2):253-9.
7
Avidity of anti-DNA antibodies in serum and IgG glomerular eluates from patients with systemic lupus erythematosus. Association of high avidity antinative DNA antibody with glomerulonephritis.系统性红斑狼疮患者血清及IgG肾小球洗脱物中抗DNA抗体的亲和力。高亲和力抗天然DNA抗体与肾小球肾炎的关联。
J Clin Invest. 1977 Jan;59(1):90-6. doi: 10.1172/JCI108626.

本文引用的文献

1
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
A quantitative immunochemical measure of the primary interaction between I BSA and antibody.一种对牛血清白蛋白（BSA）与抗体之间主要相互作用的定量免疫化学测定方法。
J Infect Dis. 1958 Nov-Dec;103(3):239-62. doi: 10.1093/infdis/103.3.239.
3
Heterogeneity and average combining constants of antibodies from individual rabbits.来自个体兔子的抗体的异质性和平均结合常数。
J Immunol. 1958 Jun;80(6):417-28.
4
Determination of antibody-hapten equilibrium constants by an ammonium sulfate precipitation technique.用硫酸铵沉淀技术测定抗体-半抗原平衡常数。
J Immunol. 1969 Sep;103(3):625-7.
5
Determination of avidity of anti-albumin antibodies in the mouse. Influence of the number of cells transferred on the quality of the secondary adoptive response.小鼠抗白蛋白抗体亲和力的测定。转移细胞数量对二次过继性反应质量的影响。
Immunology. 1969 Aug;17(2):189-98.
6
Confidence intervals for affinity constants, heterogeneity indices and concentration of reactive sites estimated by the 50 percent ammonium sulphate method on rat's anti-DNP sera.
Immunochemistry. 1972 Jul;9(7):699-707. doi: 10.1016/0019-2791(72)90014-6.
7
The use of ammonium sulphate globulin precipitation for determination of affinity of anti-protein antibodies in mouse serum.使用硫酸铵球蛋白沉淀法测定小鼠血清中抗蛋白抗体的亲和力。
Immunology. 1972 May;22(5):747-56.
8
Determination of relative affinity and heterogeneity of mouse anti-DNP antibodies by a plaque-inhibition technique.用噬斑抑制技术测定小鼠抗二硝基苯酚抗体的相对亲和力和异质性。
J Immunol. 1972 Jul;109(1):74-83.
9
Separation and characterization of birch pollen antigens with special reference to the allergenic components.
Int Arch Allergy Appl Immunol. 1972;42(3):329-42. doi: 10.1159/000230616.
10
The primary and secondary antibody response to Escherichia coli O6 lipopolysaccharide analysed at the humoral and cellular level. Amount and avidity of the antibodies in relation to protective capacity.在体液和细胞水平分析对大肠杆菌O6脂多糖的初次和二次抗体反应。抗体的数量和亲和力与保护能力的关系。
Immunology. 1973 Feb;24(2):191-202.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验