用于估计抗体量和亲和力的硫酸铵沉淀技术的有效性。

The validity of the ammonium sulphate precipitation technique of estimation of antibody amount and avidity.

作者信息

Ahlstedt S, Holmgren J, Hanson L A

出版信息

Immunology. 1973 Dec;25(6):917-22.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1422995/

Abstract

Determinations of antibody titres with the ammonium sulphate precipitation technique showed an accuracy of ± 10 per cent (<0.05). Calculation of antibody avidities with Sips' distribution formula, using suitably diluted antisera, gave a ten times greater accuracy than either undiluted or too highly diluted antisera. The accuracy was lower with the formula of Celada, Schmidt and Strom (1969) probably due to the narrow antigen ranges employed. Comparison of avidity calculation according to Sips (1948) and Celada . (1969) showed a good correlation ( = 0.73–0.79; <0.01). No correlation between antibody titre and antibody avidity values in the sera ( = 0.03–0.19) was observed.

摘要

采用硫酸铵沉淀技术测定抗体效价，其准确度为±10%（<0.05）。使用适当稀释的抗血清，根据西普斯分布公式计算抗体亲和力，其准确度比未稀释或过度稀释的抗血清高出十倍。使用塞拉达、施密特和施特罗姆（1969年）公式计算时，准确度较低，这可能是由于所采用的抗原范围较窄。根据西普斯（1948年）和塞拉达（1969年）方法计算亲和力的比较显示出良好的相关性（r = 0.73 - 0.79；<0.01）。未观察到血清中抗体效价与抗体亲和力值之间存在相关性（r = 0.03 - 0.19）。

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