实验性肾盂肾炎中的局部抗体产生：数量、亲和力及免疫球蛋白类别
Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class.
作者信息
Smith J, Holmgren J, Ahlstedt S, Hanson L A
出版信息
Infect Immun. 1974 Sep;10(3):411-5. doi: 10.1128/iai.10.3.411-415.1974.
Abstract
LOCAL ANTIBODY FORMATION IN INFECTED RABBIT KIDNEYS WAS STUDIED WITH THREE TECHNIQUES
the ammonium sulfate precipitation technique, the enzyme-linked immunosorbent assay, and by binding of newly synthesized (14)C-labeled antibodies to heat-killed bacteria. Local antibody was detected by day 11 of infection with all three techniques, and a significant correlation was found in titers by all three methods. In these studies, antibody synthesized early was in IgG and IgA class, whereas IgM antibodies appeared later (day 20) in the antibody response. No maturation of avidity of local antibody was noted with time. Since it was necessary to use different animals at each test occasion, individual differences in avidity could account for failure to note an increase in avidity with time.
摘要
采用三种技术研究了感染兔肾脏中的局部抗体形成
硫酸铵沉淀技术、酶联免疫吸附测定法以及将新合成的（14）C标记抗体与热灭活细菌结合。在感染第11天时，通过所有这三种技术均检测到局部抗体，并且所有这三种方法在滴度上发现了显著相关性。在这些研究中，早期合成的抗体属于IgG和IgA类，而IgM抗体在抗体反应后期（第20天）出现。未观察到局部抗体的亲和力随时间成熟。由于在每个测试场合都需要使用不同的动物，亲和力的个体差异可能是未能观察到亲和力随时间增加的原因。
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