Veldkamp J, van der Gaag R, Willers J M
Immunology. 1973 Nov;25(5):761-71.
The role of Peyer's patches in primary and secondary immune response was studied with as the antigen. E1 Tor ME 7 is a typical enteric antigen which was shown to be suitable for the demonstration of antibody forming cells by localized lysis in agar. No plaque-forming cells (PFC) against this antigen were found in the organs of non-immunized mice. Consequently a primary immune response could be studied. an immune response against this antigen could only be obtained with spleen cells when the donor animal had been primed. After primary intraperitoneal or enteral immunization many PFC were found in the spleen but none in Peyer's patches. This cannot be explained by insufficient penetration of the antigen into Peyer's patches as secondary responses were obtained with both routes of immunization. After local injection of antigen directly into Peyer's patches, PFC only appeared after 5 days. This is an indication that an essential cell type for the immune response is lacking in Peyer's patches. The cells from Peyer's patches were unable to restore the immune response in lethally irradiated mice, unless they were injected together with thymus cells or cells from peripheral lymph nodes. This suggests the absence of active T cells and indicates the presence of B cells in Peyer's patches. The combination of thymus cells and bone marrow cells was inactive in restoring the immune response. This is an indication that the B cells in Peyer's patches are immunologically more mature than bone marrow cells.
以E1 Tor ME 7作为抗原，研究了派尔集合淋巴结在初次和二次免疫反应中的作用。E1 Tor ME 7是一种典型的肠道抗原，已证明其适合通过琼脂中的局部溶解来展示抗体形成细胞。在未免疫小鼠的器官中未发现针对该抗原的空斑形成细胞（PFC）。因此，可以研究初次免疫反应。只有当供体动物已被致敏时，用脾细胞才能获得针对该抗原的免疫反应。初次腹腔内或肠道免疫后，在脾脏中发现了许多PFC，但在派尔集合淋巴结中未发现。这不能用抗原向派尔集合淋巴结的渗透不足来解释，因为两种免疫途径都获得了二次反应。将抗原直接局部注射到派尔集合淋巴结后，PFC仅在5天后出现。这表明派尔集合淋巴结中缺乏免疫反应所必需的细胞类型。派尔集合淋巴结的细胞无法恢复致死性照射小鼠的免疫反应，除非它们与胸腺细胞或外周淋巴结的细胞一起注射。这表明派尔集合淋巴结中缺乏活性T细胞，并表明存在B细胞。胸腺细胞和骨髓细胞的组合在恢复免疫反应方面没有活性。这表明派尔集合淋巴结中的B细胞在免疫上比骨髓细胞更成熟。
