Snippe H, Belder M, Willers J M
Immunology. 1977 Dec;33(6):931-36.
Immunization of mice with antigen mixed with cationic surface active lipid dimethyl dioctadecyl ammonium bromide (DDA) produced delayed type hypersensitivity (DH), measured as a footpad swelling. The DH to sheep red blood cells or dinitrophenyl conjugated with bovine serum albumin (DNP28-BSA) in DDA exceeded the response of the same antigens in Freund's Complete Adjuvant (FCA) significantly. Treatment of mice with CY 8 hr prior to the injection of antigen in FCA or DDA resulted in delay of the onset of footpad swelling past day 5 and in elimination of the differences in the response due to the adjuvants. Immunization with carrier or hapten-carrier complexes with different epitope density in DDA and elicitation with the homologous and heterologous antigens revealed that the DH was DNP-specific. In vivo priming with DNP28-BSA in DDA and in vitro stimulation with the same antigen resulted in peak responses which were twice as high and were reached almost twice as fast as the earlier found response following immunization in FCA. The advantages of DDA as adjuvant over covalently linked fatty acid chains and over FCA are discussed.
用与阳离子表面活性脂质二甲基二十八烷基溴化铵（DDA）混合的抗原免疫小鼠会产生迟发型超敏反应（DH），通过足垫肿胀来衡量。在DDA中，对绵羊红细胞或与牛血清白蛋白偶联的二硝基苯基（DNP28 - BSA）的DH显著超过了在弗氏完全佐剂（FCA）中相同抗原的反应。在FCA或DDA中注射抗原前8小时用环磷酰胺（CY）处理小鼠，导致足垫肿胀的起始延迟超过第5天，并消除了由于佐剂导致的反应差异。用在DDA中具有不同表位密度的载体或半抗原 - 载体复合物免疫并用同源和异源抗原激发，结果显示DH是DNP特异性的。在DDA中用DNP28 - BSA进行体内致敏并在体外用相同抗原刺激，产生的峰值反应是之前在FCA中免疫后发现的反应的两倍高，且达到峰值的速度几乎快两倍。讨论了DDA作为佐剂相对于共价连接的脂肪酸链和FCA的优势。
