Provonchee R B, Zinner S H
Appl Microbiol. 1974 Jan;27(1):185-6. doi: 10.1128/am.27.1.185-186.1974.
To screen large numbers of sera, a method was devised which utilizes the Steers-Foltz replicator which is usually used to determine minimal inhibitory concentration for antibiotics. Each of the wells (9 by 15 mm) of the replicator is filled with 0.06 ml of serum, 0.02 ml of a 10(5) suspension of organisms, and 0.02 ml of diluent (tris(hydroxymethyl)aminomethane-hydrochloride buffer, pH 8.4). The mixtures are incubated for 3 h, and samples are taken at 0, 1, 2, and 3 h by stamping duplicate nutrient agar plates (approximately 0.04 ml from each well). Plates are incubated overnight, and bactericidal activity is estimated by visual inspection of bacterial growth at each site for each sampling time. Results obtained with 28 serum-organism pairs paralleled standard pipetting-pour plate methods. The replicator method for determining bactericidal activity allows for the testing of a large number of samples and requires negligible amounts of serum.
为了筛选大量血清，设计了一种方法，该方法利用通常用于测定抗生素最小抑菌浓度的施特斯 - 福尔茨复制器。复制器的每个孔（9×15毫米）中装入0.06毫升血清、0.02毫升10⁵浓度的菌悬液和0.02毫升稀释剂（三（羟甲基）氨基甲烷 - 盐酸缓冲液，pH 8.4）。混合物孵育3小时，在0、1、2和3小时通过冲压一式两份的营养琼脂平板（每个孔约0.04毫升）取样。平板过夜孵育，通过目视检查每个取样时间每个位点的细菌生长来估计杀菌活性。用28对血清 - 菌株组合获得的结果与标准移液 - 倾注平板法平行。用于测定杀菌活性的复制器方法允许测试大量样品，并且所需血清量可忽略不计。
