Domingue G J, Neter E
J Bacteriol. 1966 Jan;91(1):129-33. doi: 10.1128/jb.91.1.129-133.1966.
Domingue, Gerald J. (State University of New York at Buffalo, Buffalo, N.Y.), and Erwin Neter. Opsonizing and bactericidal activity of antibodies against common antigen of Enterobacteriaceae. J. Bacteriol. 91:129-133. 1966.-In addition to the well-known O, H, K, and Vi antigens, Enterobacteriaceae produce a common antigen, which was first identified by hemagglutination tests with Escherichia coli O14 antiserum. Studies on the biological significance of this antigen by in vitro phagocytic experiments have revealed that opsonization is markedly enhanced in the presence of the corresponding antibody, rabbit polymorphonuclear leukocytes, and normal rabbit serum. This effect is specific, because Pseudomonas aeruginosa, which is devoid of this antigen, is not opsonized under these conditions, and removal of the antibody by absorption markedly reduces the uptake of enteric bacteria containing the antigen. Opsonization thus represents another method for the study of this antigen-antibody system. Bactericidal tests have revealed that antibodies against this antigen, engendered in rabbits by different strains of enteric bacteria and various procedures, are bactericidal for E. coli O14 but not for other enteric bacteria, possibly due to previously demonstrated differences between the antigen moieties obtained from these microorganisms.
多明格，杰拉尔德·J.（纽约州立大学布法罗分校，纽约州布法罗），以及欧文·内特尔。抗肠杆菌科共同抗原抗体的调理和杀菌活性。《细菌学杂志》91:129 - 133。1966年。——除了众所周知的O、H、K和Vi抗原外，肠杆菌科还产生一种共同抗原，该抗原最初是通过用大肠杆菌O14抗血清进行血凝试验鉴定出来的。通过体外吞噬实验对该抗原生物学意义的研究表明，在相应抗体、兔多形核白细胞和正常兔血清存在的情况下，调理作用会显著增强。这种效应具有特异性，因为缺乏这种抗原的铜绿假单胞菌在这些条件下不会被调理，通过吸收去除抗体则会显著降低含有该抗原的肠道细菌的摄取。因此，调理作用代表了研究这种抗原 - 抗体系统的另一种方法。杀菌试验表明，由不同菌株的肠道细菌和各种程序在兔体内产生的针对该抗原的抗体，对大肠杆菌O14具有杀菌作用，但对其他肠道细菌则没有，这可能是由于先前证明的从这些微生物获得的抗原部分之间存在差异。
