酵母线粒体中rRNA的突变研究：大rRNA基因中T1696的功能重要性。
Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.
作者信息
Daignan-Fornier B, Bolotin-Fukuhara M
机构信息
Laboratoire de Génétique Moléculaire, Université Paris-Sud, Orsay, France.
出版信息
Nucleic Acids Res. 1988 Oct 11;16(19):9299-306. doi: 10.1093/nar/16.19.9299.
Abstract
Four intragenic suppressors of a mitochondrial mutation in the 21S rRNA gene have been characterized in S. cerevisiae. The determination of the nature of the nucleotide changes in the suppressor strains showed that a T at position 1696 in the large rRNA gene is essential for correct function of the mitoribosome. The importance of this specific nucleotide and the fact that this mitochondrial mutation can also be suppressed by a mutation in a nuclear gene are in good agreement with a rRNA-r protein interaction in this part of domain IV, which functional importance is demonstrated in vivo by our results.
摘要
在酿酒酵母中已鉴定出21S rRNA基因中线粒体突变的四个基因内抑制子。对抑制菌株中核苷酸变化性质的测定表明，大rRNA基因中第1696位的T对于线粒体核糖体的正确功能至关重要。这个特定核苷酸的重要性以及该线粒体突变也可被核基因中的突变抑制这一事实，与结构域IV这一部分中的rRNA-r蛋白相互作用高度一致，我们的结果在体内证明了其功能重要性。
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