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1
Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.两种蛋白质因子和ATP参与大肠杆菌DNA聚合酶3催化的体外DNA合成。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6.
2
Conversion of phichi174 and fd DNA to their replicative forms by two enzyme systems in Escherichia coli.通过大肠杆菌中的两种酶系统将噬菌体φX174和fd DNA转化为它们的复制形式。
Basic Life Sci. 1974;3:395-409. doi: 10.1007/978-1-4613-4529-9_31.
3
Studies on in vitro DNA synthesis. II. Isolation of a protein which stimulates deoxynucleotide incorporation catalyzed by DNA polymerase of E. coli.体外DNA合成研究。II. 一种刺激大肠杆菌DNA聚合酶催化的脱氧核苷酸掺入的蛋白质的分离。
Biochem Biophys Res Commun. 1973 Mar 17;51(2):257-67. doi: 10.1016/0006-291x(73)91251-5.
4
DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein.T4噬菌体DNA聚合酶和T4基因32解旋蛋白在双链DNA模板上进行DNA合成。
J Biol Chem. 1974 Sep 10;249(17):5668-76.
5
Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid in ribonucleic acid-deoxyribonucleic acid hybrid structures.从大肠杆菌中分离并鉴定一种对核糖核酸 - 脱氧核糖核酸杂交结构中的核糖核酸具有特异性的核酸内切酶。
J Biol Chem. 1973 Sep 10;248(17):5914-21.
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Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.通过纯化蛋白将M13病毒单链转化为双链复制形式。
J Biol Chem. 1974 Jul 10;249(13):3999-4005.
7
A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.从大肠杆菌中分离出的一种DNA解旋蛋白:它与DNA及DNA聚合酶的相互作用。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41. doi: 10.1073/pnas.69.12.3537.
8
Stimulation of T7 DNA polymerase by a new phage-coded protein.一种新的噬菌体编码蛋白对T7 DNA聚合酶的刺激作用。
Mol Gen Genet. 1973 Jul 2;123(3):247-62. doi: 10.1007/BF00271243.
9
The mechanism of replication of phi X174 single-stranded DNA. VI. Requirements for ribonucleoside triphosphates and DNA polymerase 3.φX174单链DNA的复制机制。VI. 核糖核苷三磷酸和DNA聚合酶3的需求
Can J Biochem. 1973 Dec;51(12):1588-97. doi: 10.1139/o73-214.
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Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.

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Prioritization of causal genes for coronary artery disease based on cumulative evidence from experimental and in silico studies.基于实验和计算研究的累积证据对冠心病因果基因进行优先级排序。
Sci Rep. 2020 Jun 26;10(1):10486. doi: 10.1038/s41598-020-67001-w.
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Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic ψχ heterodimer.大肠杆菌 DNA 聚合酶 III 夹取器的解决方案研究揭示了动态 ψχ 异二聚体的位置。
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Interactions of the Escherichia coli primosomal PriB protein with the single-stranded DNA. Stoichiometries, intrinsic affinities, cooperativities, and base specificities.大肠杆菌 Primosomal PriB 蛋白与单链 DNA 的相互作用。化学计量学、固有亲和力、协同性和碱基特异性。
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Loading clamps for DNA replication and repair.用于DNA复制和修复的加载夹。
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Elongation of primed DNA templates by eukaryotic DNA polymerases.真核生物DNA聚合酶对引发的DNA模板的延伸。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5827-31. doi: 10.1073/pnas.77.10.5827.
7
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
8
DNA polymerase III holoenzyme of Escherichia coli: components and function of a true replicative complex.大肠杆菌DNA聚合酶III全酶:真正复制复合体的组成成分与功能
Mol Cell Biochem. 1985 Feb;66(1):71-85. doi: 10.1007/BF00231826.
9
Relation of the Escherichia coli dnaX gene to its two products--the tau and gamma subunits of DNA polymerase III holoenzyme.大肠杆菌 dnaX 基因与其两种产物——DNA 聚合酶 III 全酶的 τ 亚基和 γ 亚基的关系。
Nucleic Acids Res. 1987 Oct 12;15(19):7663-75. doi: 10.1093/nar/15.19.7663.
10
Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerases alpha and delta.人单链DNA结合蛋白在猴病毒40 DNA复制中的多种功能:单链稳定以及对DNA聚合酶α和δ的刺激作用
Proc Natl Acad Sci U S A. 1989 Dec;86(24):9757-61. doi: 10.1073/pnas.86.24.9757.

本文引用的文献

1
CHARACTERIZATION OF A RIBOSOME-LINKED GUANOSINE TRIPHOSPHATASE IN ESCHERICHIA COLI EXTRACTS.大肠杆菌提取物中一种核糖体连接的鸟苷三磷酸酶的特性分析
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1462-9. doi: 10.1073/pnas.52.6.1462.
2
AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS.一种用于制备温度调节聚丙烯酰胺凝胶电泳的装置。
Anal Biochem. 1964 Nov;9:351-69. doi: 10.1016/0003-2697(64)90192-7.
3
Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.在单链DNA-琼脂糖柱上对DNA结合酶进行亲和层析。
Eur J Biochem. 1972 Apr 24;26(4):474-81. doi: 10.1111/j.1432-1033.1972.tb01789.x.
4
Function of DNA polymerase 3 in DNA replication.DNA聚合酶3在DNA复制中的功能。
Nat New Biol. 1971 Dec 29;234(52):285-6. doi: 10.1038/newbio234285a0.
5
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
6
Replication and repair of DNA in cells of Escherichia coli treated with toluene.用甲苯处理的大肠杆菌细胞中DNA的复制与修复
Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81. doi: 10.1073/pnas.67.2.674.
7
DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells.核苷酸可通透的大肠杆菌细胞中的DNA合成。I. 经乙醚处理的细胞的制备及特性
J Mol Biol. 1971 Jun 28;58(3):739-53. doi: 10.1016/0022-2836(71)90037-4.
8
Escherichia coli mutants with temperature-sensitive synthesis of DNA.具有温度敏感型DNA合成能力的大肠杆菌突变体。
Mol Gen Genet. 1970;109(2):107-22. doi: 10.1007/BF00269647.
9
A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template.一种新型DNA聚合酶3和一种共聚合酶复制一条长的单链引物模板。
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1764-7. doi: 10.1073/pnas.70.6.1764.
10
Purification and properties of DNA polymerase 3.DNA聚合酶3的纯化及特性
Eur J Biochem. 1973 May 2;34(3):440-7. doi: 10.1111/j.1432-1033.1973.tb02777.x.

两种蛋白质因子和ATP参与大肠杆菌DNA聚合酶3催化的体外DNA合成。

Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.

作者信息

Hurwitz J, Wickner S

出版信息

Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6.

DOI:10.1073/pnas.71.1.6
PMID:4589895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC387920/
Abstract

The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg(+2), spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.

摘要

噬菌体fd单链DNA在体外转变为双链结构取决于大肠杆菌RNA聚合酶、DNA聚合酶III、核糖核苷三磷酸和脱氧核糖核苷三磷酸、Mg(+2)、亚精胺或大肠杆菌的DNA解旋蛋白,以及另外两种蛋白质因子,在此称为因子I和因子II。这两种因子对于DNA聚合酶III催化的、依赖于聚(dA)·寡聚(dT)引物模板的dTMP掺入也是必不可少的。在后一反应中,绝对依赖于ATP或dATP。