两种蛋白质因子和ATP参与大肠杆菌DNA聚合酶3催化的体外DNA合成。
Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.
作者信息
Hurwitz J, Wickner S
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10. doi: 10.1073/pnas.71.1.6.
Abstract
The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg(+2), spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.
摘要
噬菌体fd单链DNA在体外转变为双链结构取决于大肠杆菌RNA聚合酶、DNA聚合酶III、核糖核苷三磷酸和脱氧核糖核苷三磷酸、Mg(+2)、亚精胺或大肠杆菌的DNA解旋蛋白，以及另外两种蛋白质因子，在此称为因子I和因子II。这两种因子对于DNA聚合酶III催化的、依赖于聚（dA）·寡聚（dT）引物模板的dTMP掺入也是必不可少的。在后一反应中，绝对依赖于ATP或dATP。
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AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS.一种用于制备温度调节聚丙烯酰胺凝胶电泳的装置。
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