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某些烷基化致癌物的产物O6-甲基鸟嘌呤的细胞反应。

Cellular reactions of O6-methylguanine, a product of some alkylating carcinogens.

作者信息

Miller C T, Lawley P D, Shah S A

出版信息

Biochem J. 1973 Oct;136(2):387-93. doi: 10.1042/bj1360387.

DOI:10.1042/bj1360387
PMID:4590203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165965/
Abstract

Cultures of a purine-requiring mutant of Chinese hamster ovary cells (CHO-104b), randomly bred hamster embryo cells, or Escherichia coli B(s-1) were treated with non-toxic doses of (3)H-labelled O(6)-methylguanine. DNA and RNA were isolated and subjected to enzymic digestion to nucleosides at pH8. The products of digestion were analysed by ion-exchange chromatography on columns of Dowex 50 (NH(4) (+) form) at pH8.9. No (3)H-labelled O(6)-methylguanosine was detected in nucleic acid digests. (3)H-labelled O(6)-methylguanine was O-demethylated yielding [(3)H]guanine in CHO-104b cells. Radioactivity in nucleic acid digests was associated with thymidine, guanosine, deoxyguanosine and an unidentified early-eluting product. Reports of similar unidentified products from nucleic acids labelled with various agents are discussed.

摘要

用无毒剂量的³H标记的O⁶-甲基鸟嘌呤处理中国仓鼠卵巢细胞（CHO-104b）的嘌呤需求突变体培养物、随机繁殖的仓鼠胚胎细胞或大肠杆菌B(s-1)。分离DNA和RNA，并在pH8条件下进行酶促消化成核苷。消化产物在pH8.9条件下通过Dowex 50（NH₄⁺形式）柱进行离子交换色谱分析。在核酸消化物中未检测到³H标记的O⁶-甲基鸟苷。在CHO-104b细胞中，³H标记的O⁶-甲基鸟嘌呤发生O-去甲基化，产生[³H]鸟嘌呤。核酸消化物中的放射性与胸苷、鸟苷、脱氧鸟苷和一种未鉴定的早期洗脱产物有关。讨论了用各种试剂标记核酸产生类似未鉴定产物的报道。

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本文引用的文献

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DEMETHYLATION OF PURINE ANALOGS BY MICROSOMAL ENZYMES FROM MOUSE LIVER.小鼠肝脏微粒体酶对嘌呤类似物的去甲基化作用
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Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides.脱氧鸟苷的O-6烷基化与亚硝胺和亚硝酰胺的致突变性和致癌性之间可能存在的关联。
Nature. 1969 Jul 12;223(5202):206-7. doi: 10.1038/223206a0.
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Tissue-dependent differences in DNA methylation products of mice treated with methyl-labelled methylnitrosourea.用甲基标记的甲基亚硝基脲处理的小鼠DNA甲基化产物的组织依赖性差异。
Int J Cancer. 1971 May 15;7(3):436-42. doi: 10.1002/ijc.2910070309.
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