性因子F转移起始位点的定位
Location of the origin of transfer of the sex factor F.
作者信息
Willetts N S
出版信息
J Bacteriol. 1972 Nov;112(2):773-8. doi: 10.1128/jb.112.2.773-778.1972.
Abstract
The location of the origin of transfer on the map of Flac has been determined by two different techniques. The first showed that no transfer cistrons were transferred early by a transposition Hfr strain with a known point of chromosomal integration into Flac, and the second used a series of transfer-deficient Hfr deletion strains to map a locus required in cis for chromosome transfer by an incoming Flac factor. Both techniques gave results consistent with a location for the origin of transfer between traJ and the phi(R) (11) locus.
摘要
在Flac图谱上转移起点的位置已通过两种不同技术确定。第一种方法表明，具有已知染色体整合点并导入Flac的转座高频重组（Hfr）菌株不会早期转移任何转移顺反子；第二种方法使用了一系列转移缺陷型Hfr缺失菌株，来定位由导入的Flac因子进行染色体转移时顺式所需的一个位点。两种技术所得结果均与转移起点位于traJ和phi(R)(11)位点之间的位置相符。
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