Brooks S J, Lyons J M, Braude A I
Am J Pathol. 1974 Feb;74(2):345-58.
Retrograde pyelonephritis was produced in rats by introducing into the bladder a small refluxing inoculum of Escherichia coli that would enter the renal pelvis but not the blood stream. At the same time the left ureter was partially obstructed for 18 hours. This model differs from previous attempts to produce E coli pyelonephritis with large (0.6 ml) volumes infused into the bladder, because such large volumes cause bacteremia and hematogeneous pyelonephritis. Since retrograde E coli pyelonephritis in patients is not accompanied by positive blood cultures, the model described in this report is believed to accurately mimic human pyelonephritis and to allow a realistic approach to the study of immunity against retrograde infection in the urinary tract.
通过向大鼠膀胱内注入少量能反流进入肾盂但不进入血流的大肠杆菌接种物，诱发大鼠发生逆行性肾盂肾炎。与此同时，左输尿管被部分阻塞18小时。该模型不同于以往通过向膀胱内注入大量（0.6毫升）大肠杆菌来诱发肾盂肾炎的尝试，因为如此大量会导致菌血症和血源性肾盂肾炎。由于人类患者的逆行性大肠杆菌肾盂肾炎不伴有血培养阳性，本报告中描述的模型被认为能准确模拟人类肾盂肾炎，并为研究针对尿路逆行感染的免疫提供一种切实可行的方法。
