• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Magic spot metabolism in an Escherichia coli mutant temperature sensitive in elongation factor Ts.在延伸因子Ts温度敏感的大肠杆菌突变体中的魔斑代谢
J Bacteriol. 1974 Mar;117(3):1195-200. doi: 10.1128/jb.117.3.1195-1200.1974.
2
Synthesis of stable RNA in stringent Escherichia coli cells in the absence of charged transfer RNA.在缺乏带电荷转运RNA的情况下，严谨型大肠杆菌细胞中稳定RNA的合成。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):689-92. doi: 10.1073/pnas.70.3.689.
3
Inhibition of stable RNA synthesis by levallorphan in Escherichia coli. Implication of compounds MS I and MS II.左洛啡烷对大肠杆菌中稳定RNA合成的抑制作用。化合物MS I和MS II的影响。
Eur J Biochem. 1973 Dec 3;40(1):31-42. doi: 10.1111/j.1432-1033.1973.tb03166.x.
4
Specific recognition of GTpsiC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli.来自大肠杆菌的50S核糖体亚基对tRNA的GTpsiC环（环IV）的特异性识别。
Nat New Biol. 1973 Dec 5;246(153):132-5. doi: 10.1038/newbio246132a0.
5
Regulation of intracellular protein breakdown in stringent and relaxed strains of E. coli,大肠杆菌严谨型和松弛型菌株中细胞内蛋白质降解的调控
Cell. 1974 May;2(1):31-5. doi: 10.1016/0092-8674(74)90005-1.
6
Characterization of mutants of Escherichia coli temperature-sensitive for ribonucleic acid regulation: an unusual phenotype associated with a phenylalanyl transfer ribonucleic acid synthetase mutant.对核糖核酸调节温度敏感的大肠杆菌突变体的特性研究：与苯丙氨酰转移核糖核酸合成酶突变体相关的一种异常表型。
J Bacteriol. 1971 Nov;108(2):627-38. doi: 10.1128/jb.108.2.627-638.1971.
7
Guanosine 5'-diphosphate 3'-diphosphate levels, carbon source, and ribonucleic acid synthesis in a mutant strain of Escherichia coli.大肠杆菌突变株中的鸟苷5'-二磷酸3'-二磷酸水平、碳源与核糖核酸合成
Biochemistry. 1983 Mar 1;22(5):1123-8. doi: 10.1021/bi00274a020.
8
Control of RNA synthesis in Escherichia coli. IV. Effect of levallorphan on guanosine 3'-diphosphate 5'-diphosphate metabolism in spoT+ and spoT minus strains.大肠杆菌中RNA合成的调控。IV. 左洛啡烷对spoT+和spoT-菌株中二磷酸鸟苷-5'-二磷酸代谢的影响。
Biochim Biophys Acta. 1974 Oct 28;366(3):279-87.
9
Guanine nucleotide metabolism in a mutant strain of Escherichia coli with a temperature sensitive lesion in rRNA synthesis.在核糖体RNA合成中存在温度敏感损伤的大肠杆菌突变株中的鸟嘌呤核苷酸代谢。
Biochim Biophys Acta. 1978 Dec 21;521(2):634-40. doi: 10.1016/0005-2787(78)90304-0.
10
Role of guanine nucleotides in protein synthesis. Elongation factor G and guanosine 5'-triphosphate,3'-diphosphate.鸟嘌呤核苷酸在蛋白质合成中的作用。延伸因子G与鸟苷5'-三磷酸、3'-二磷酸
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3250-4. doi: 10.1073/pnas.70.11.3250.

引用本文的文献

1
Bacillus subtilis mutant temperature sensitive in the synthesis of ribonucleic acid.在核糖核酸合成中对温度敏感的枯草芽孢杆菌突变体。
J Bacteriol. 1976 Aug;127(2):679-90. doi: 10.1128/jb.127.2.679-690.1976.
2
Inhibition of leucyl-transfer ribonucleic acid synthetasymol.亮氨酰转移核糖核酸合成酶的抑制作用
Biochem J. 1975 Dec;152(3):511-5. doi: 10.1042/bj1520511.
3
Inhibition of isoleucyl-transfer ribonucleic acid synthetase in Escherichia coli by pseudomonic acid.假单胞菌酸对大肠杆菌异亮氨酰 - 转移核糖核酸合成酶的抑制作用
Biochem J. 1978 Oct 15;176(1):305-18. doi: 10.1042/bj1760305.
4
A study of a mutant elongation factor properties of E. coli HAK88 and its mutant elongation factor Tu.大肠杆菌HAK88及其突变延伸因子Tu的突变延伸因子特性研究。
Mol Gen Genet. 1978 Feb 7;159(1):47-55. doi: 10.1007/BF00401747.
5
Genetic analysis of mutations affecting ribonuclease II in Escherichia coli.
Mol Gen Genet. 1977 May 20;153(1):1-4. doi: 10.1007/BF01035989.
6
Effects of some antibiotics on the stringent control of RNA synthesis in Escherichia coli.某些抗生素对大肠杆菌中RNA合成严谨控制的影响。
Mol Gen Genet. 1975 Sep 8;139(4):321-7. doi: 10.1007/BF00267972.

本文引用的文献

1
Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp.密码子特异性、依赖tRNA的体外合成ppGpp和pppGpp。
Nat New Biol. 1973 May 2;243(122):13-5.
2
PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES.具有温度敏感型氨酰核糖核酸合成酶的大肠杆菌两个突变体中的蛋白质和核酸合成
J Bacteriol. 1965 Mar;89(3):706-11. doi: 10.1128/jb.89.3.706-711.1965.
3
Codon-specific binding of deacylated transfer ribonucleic acid to ribosomes.脱酰化转移核糖核酸与核糖体的密码子特异性结合。
J Biol Chem. 1970 Jun;245(12):3195-202.
4
Isolation and characterization of ribonuclease I mutants of Escherichia coli.大肠杆菌核糖核酸酶I突变体的分离与鉴定
J Mol Biol. 1966 Mar;16(1):67-84. doi: 10.1016/s0022-2836(66)80263-2.
5
Inhibitors of ribosome functions.核糖体功能抑制剂。
Annu Rev Microbiol. 1971;25:487-562. doi: 10.1146/annurev.mi.25.100171.002415.
6
On the regulation of guanosine tetraphosphate levels in stringent and relaxed strains of Escherichia coli.关于大肠杆菌严谨型和松弛型菌株中鸟苷四磷酸水平的调控
J Biol Chem. 1971 Jul 25;246(14):4381-5.
7
Factor necessary for ribosomal RNA synthesis.核糖体RNA合成所需的因子。
Nature. 1970 Nov 21;228(5273):748-51. doi: 10.1038/228748a0.
8
Characterization of altered forms of glycyl transfer ribonucleic acid synthetase and the effects of such alterations on aminoacyl transfer ribonucleic acid synthesis in vivo.甘氨酰转移核糖核酸合成酶改变形式的表征及其在体内对氨酰基转移核糖核酸合成的影响。
J Bacteriol. 1970 Apr;102(1):204-12. doi: 10.1128/jb.102.1.204-212.1970.
9
Mechanism of protein biosynthesis.蛋白质生物合成机制。
Bacteriol Rev. 1969 Jun;33(2):264-301. doi: 10.1128/br.33.2.264-301.1969.
10
Amino acid control over RNA synthesis: a re-evaluation.氨基酸对RNA合成的控制：重新评估
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1345-52. doi: 10.1073/pnas.60.4.1345.

在延伸因子Ts温度敏感的大肠杆菌突变体中的魔斑代谢

Magic spot metabolism in an Escherichia coli mutant temperature sensitive in elongation factor Ts.

作者信息

Glazier K, Schlessinger D

出版信息

J Bacteriol. 1974 Mar;117(3):1195-200. doi: 10.1128/jb.117.3.1195-1200.1974.

DOI:10.1128/jb.117.3.1195-1200.1974
PMID:4591947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC246601/
Abstract

A temperature-sensitive mutant of Escherichia coli HAK88 which has been shown to have a lesion in elongation factor Ts (EFTs) was studied with repsect to its metabolism of guanosine 5'-diphosphate, 2'(3')-diphosphate (ppGpp) and the associated failure of ribosomal ribonucleic acid (rRNA) accumulation at the nonpermissive temperature. Results reported here show that (i) when EFTs is nonfunctional, a full complement of charged transfer RNA (tRNA) cannot prevent accumulation of ppGpp (magic spot) and the stringent failure of rRNA accumulation; (ii) chloramphenicol prevents magic spot (MS) formation and the stringent response not by increasing the percentage of charged tRNA, but possibly by somehow interfering directly with the synthesis of MS; and (iii) tetracycline can lead to MS disappearance without resumption of RNA synthesis. Thus, the absence of MS and the presence of a functional RNA polymerase and charged tRNA are not sufficient to support rRNA accumulation in vivo. An additional element in the regulatory system is suggested.

摘要

对大肠杆菌HAK88的一个温度敏感突变体进行了研究，该突变体已被证明在延伸因子Ts（EFTs）中存在损伤，研究内容涉及其对鸟苷5'-二磷酸、2'（3'）-二磷酸（ppGpp）的代谢以及在非允许温度下核糖体核糖核酸（rRNA）积累相关的失败情况。此处报告的结果表明：（i）当EFTs无功能时，完整的带电转移RNA（tRNA）并不能阻止ppGpp（魔斑）的积累以及rRNA积累的严谨型失败；（ii）氯霉素阻止魔斑（MS）形成和严谨型反应，不是通过增加带电tRNA的比例，而是可能通过某种方式直接干扰MS的合成；（iii）四环素可导致MS消失而不恢复RNA合成。因此，MS的缺失以及功能性RNA聚合酶和带电tRNA的存在不足以支持体内rRNA的积累。提示了调节系统中的一个额外因素。