Rose R E, Geldreich E E, Litsky W
Appl Microbiol. 1975 Apr;29(4):532-6. doi: 10.1128/am.29.4.532-536.1975.
A two-layer agar method has been developed which consistently yields higher recovery of fecal coliforms on membrane filters when compared to the existing membrane fecal coliform procedure. This method has been evaluated by three laboratories using samples of raw and chlorinated waste water, and reservoir, river, and marine waters. Verification of 1,013 fecal coliform colonies isolated from 61 water samples averaged 92% on this proposed procedure. Comparison with the Standard Methods membrane fecal coliform procedure revealed the two-layer agar method had an overall increased sensitivity to fecal coliform detection in these waters. It is therefore proposed that this procedure be evaluated as an alternative to the Standard Methods fecal coliform membrane Filter test in the examination of chlorinated secondary effluents, marine waters, and any natural waters that may contain pollutants with heavy metal ions.
已开发出一种双层琼脂法，与现有的膜滤粪便大肠菌群检测方法相比，该方法能使膜滤器上粪便大肠菌群的回收率始终更高。三个实验室使用原废水和氯化废水样本以及水库水、河水和海水样本对该方法进行了评估。从61个水样中分离出的1013个粪便大肠菌群菌落，在此提议的方法上验证平均为92%。与标准方法的膜滤粪便大肠菌群检测方法相比，双层琼脂法在这些水体中对粪便大肠菌群检测的总体灵敏度有所提高。因此，建议在检测氯化二级出水、海水以及任何可能含有重金属离子污染物的天然水体时，将该方法作为标准方法粪便大肠菌群膜滤试验的替代方法进行评估。
