Tereletsky M J, Barrow W W
Infect Immun. 1983 Sep;41(3):1312-21. doi: 10.1128/iai.41.3.1312-1321.1983.
The superficial L1 layer of Mycobacterium intracellulare serovar 20 was detected by immunocytochemical procedures based on C-mycoside glycopeptidolipid (GPL) antigens. Specific immune serum was produced by injecting a GPL-methylated bovine serum albumin complex into rabbits with Freund incomplete adjuvant. The resulting antibodies were used to label serovar 20 with goat anti-rabbit immunoglobulin G (IgG) conjugated with either fluorescein or ferritin. The labeled GPL antigens were then detected by fluorescent and electron microscopy. With these procedures, it was possible to observe the superficial distribution of the GPL antigens on serovar 20 and to confirm their intraphagosomal location within the macrophages after phagocytosis. These immunocytochemical procedures now make it possible to monitor these mycobacterial antigens during phagocytosis and may be helpful in determining their role in pathogenesis.
基于C-霉菌糖苷糖脂（GPL）抗原，通过免疫细胞化学方法检测细胞内分枝杆菌血清型20的浅表L1层。通过将GPL甲基化牛血清白蛋白复合物与弗氏不完全佐剂一起注射到兔子体内来产生特异性免疫血清。所得抗体用于用与荧光素或铁蛋白偶联的山羊抗兔免疫球蛋白G（IgG）标记血清型20。然后通过荧光显微镜和电子显微镜检测标记的GPL抗原。通过这些程序，可以观察到GPL抗原在血清型20上的浅表分布，并确认吞噬后它们在巨噬细胞内的吞噬体位置。这些免疫细胞化学程序现在使得在吞噬过程中监测这些分枝杆菌抗原成为可能，并且可能有助于确定它们在发病机制中的作用。
