钼饥饿和钨对肺炎克雷伯菌中固氮酶组分合成的影响。
Effect of molybdenum starvation and tungsten on the synthesis of nitrogenase components in Klebsiella pneumonia.
作者信息
Brill W J, Steiner A L, Shah V K
出版信息
J Bacteriol. 1974 Jun;118(3):986-9. doi: 10.1128/jb.118.3.986-989.1974.
Abstract
Klebsiella pneumoniae M5a1 grows well in the presence or absence of molybdenum in media containing excess NH(4) (+). However, growth on N(2) is completely dependent on the presence of molybdenum in the medium. Tungstate competes with the molybdate requirement during growth on N(2). In molybdenum-depleted medium, neither protein component of nitrogenase is active and neither component can be detected antigenically. These data provide evidence that molybdenum is an inducer of nitrogenase synthesis.
摘要
肺炎克雷伯菌M5a1在含有过量NH(4) (+)的培养基中，无论有无钼都能良好生长。然而，在N(2)上生长则完全依赖于培养基中钼的存在。在以N(2)为氮源生长时，钨酸盐会与钼酸盐需求竞争。在钼缺乏的培养基中，固氮酶的两种蛋白质成分均无活性，且无法通过抗原检测到任何一种成分。这些数据证明钼是固氮酶合成的诱导物。
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