在铵存在的情况下，导致肺炎克雷伯菌对固氮酶合成去阻遏的固氮基因中的突变。
Mutations in nif genes that cause Klebsiella pneumoniae to be derepressed for nitrogenase synthesis in the presence of ammonium.
作者信息
MacNeil D, Brill W J
出版信息
J Bacteriol. 1980 Nov;144(2):744-51. doi: 10.1128/jb.144.2.744-751.1980.
Abstract
Four Nif+ revertants from strains with polar insertions in nifL, were insensitive to ammonium and amino acid repression of nitrogenase synthesis. These strains have mutations located in or near the nifL region. The derepressed phenotype was dominant in a merodiploid containing a nif+ plasmid. These nif regulatory mutations also suppressed the Nif- phenotype of Gln- strains. Thus, regulation by fixed nitrogen (possible via glutamine synthetase) occurs on the nifLA operon but not on the other six nif operons.
摘要
来自在nifL中有极性插入的菌株的四个Nif⁺回复突变体对固氮酶合成的铵和氨基酸阻遏不敏感。这些菌株在nifL区域内或附近发生了突变。去阻遏表型在含有nif⁺质粒的部分二倍体中占主导。这些nif调控突变也抑制了Gln⁻菌株的Nif⁻表型。因此，固定氮（可能通过谷氨酰胺合成酶）的调控发生在nifLA操纵子上，而不是其他六个nif操纵子上。
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