肺炎克雷伯菌中氧气对固氮酶合成的调控
Regulation of nitrogenase synthesis by oxygen in Klebsiella pneumoniae.
作者信息
St John R T, Shah V K, Brill W J
出版信息
J Bacteriol. 1974 Jul;119(1):266-9. doi: 10.1128/jb.119.1.266-269.1974.
Abstract
Klebsiella pneumoniae does not fix N(2) under aerobic conditions. The two protein components required for nitrogenase activity were studied during aeration of cells in nitrogen-free media. Component II of nitrogenase was inactivated more slowly in vivo than component I during aeration. The rate of loss of component II was less than the rate of component II synthesis during derepression. No inactive components were detected in cells that had been growing on NH(4) (+) and then aerated in nitrogen-free medium. This supports the hypothesis that O(2) somehow represses the formation of nitrogenase.
摘要
肺炎克雷伯菌在有氧条件下不能固定N₂。在无氮培养基中对细胞通气时，研究了固氮酶活性所需的两种蛋白质成分。通气过程中，固氮酶的组分II在体内失活比组分I更慢。去阻遏过程中，组分II的损失速率小于其合成速率。在以NH₄⁺为氮源生长然后在无氮培养基中通气的细胞中未检测到无活性成分。这支持了氧气以某种方式抑制固氮酶形成的假说。
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