固定化大肠杆菌细胞连续生产L-天冬氨酸的基础研究。
Basic studies for continuous production of L-aspartic acid by immobilized Escherichia coli cells.
作者信息
Tosa T, Sato T, Mori T, Chibata I
出版信息
Appl Microbiol. 1974 May;27(5):886-9. doi: 10.1128/am.27.5.886-889.1974.
Abstract
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg(2+) was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.
摘要
通过使用填充有固定在聚丙烯酰胺凝胶晶格中的大肠杆菌细胞的柱子，研究了从富马酸铵连续生产L-天冬氨酸的条件。当含有1 mM Mg(2+)的1 M富马酸铵溶液（pH 8.5）在37℃下以空速（SV）= 0.8的流速通过固定化细胞柱时，达到了最高反应速率。从柱流出物中，以良好的产率获得了L-天冬氨酸。固定化细胞柱非常稳定。
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本文引用的文献
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