Pattison J R, Noone P, Slack R C, Deacon S
Br J Exp Pathol. 1974 Apr;55(2):169-76.
The hydrolysis of urea by N.C.I.B. 10823 has been investigated and found to be independent of bacterial growth up to 90 minutes incubation and to require only a source of readily available amino acids. Evidence is presented which indicates that the urease of N.C.I.B. 10823 is an inducible enzyme. The induction time of the urease in stationary phase organisms is 20-30 minutes and only those antibiotics which can inhibit protein synthesis within this time (aminoglycosides, tetracycline, chloramphenicol and spectinomycin) have an inhibitory effect on the hydrolysis of urea. N.C.I.B. 10823 is found to be 8-10 times more sensitive to gentamicin at low NaCl concentrations. The significance of these findings in relation to the urease assay for aminoglycoside antibiotics is discussed.
对英国国立生物标准与控制研究所（N.C.I.B.）10823菌株水解尿素的情况进行了研究，发现直至培养90分钟，该过程均与细菌生长无关，且仅需易于获取的氨基酸来源。所提供的证据表明，N.C.I.B. 10823菌株的脲酶是一种诱导酶。在稳定期生物体中，脲酶的诱导时间为20 - 30分钟，只有那些能在此时间段内抑制蛋白质合成的抗生素（氨基糖苷类、四环素、氯霉素和壮观霉素）才会对尿素水解产生抑制作用。发现N.C.I.B. 10823菌株在低氯化钠浓度下对庆大霉素的敏感性要高8 - 10倍。讨论了这些发现对于氨基糖苷类抗生素脲酶测定的意义。
