Chessin L N, Börjeson J, Welsh P D, Douglas S D, Cooper H L
J Exp Med. 1966 Nov 1;124(5):873-84. doi: 10.1084/jem.124.5.873.
A study of the kinetics of RNA and DNA synthesis in PWM-stimulated lymphocytes revealed that RNA synthesis preceded the onset of DNA synthesis by approximately 24 hr and that DNA synthesis and transformation was maximal between 66 to 78 hr. Histochemical and radioautographic studies on PWM stimulated cultures indicated that at 72 hr 50 to 60% of the cell population had been transformed by PWM, and that a distinct cell type bearing cytologic resemblance to the early plasma cell had emerged. The RNA sedimentation profile for newly synthesized RNA in PWM-stimulated cells showed that a large peak of 45 to 50 S material was formed after 24 and 40 hr. PWM thus produces a distinctive transformation of human peripheral blood lymphocytes.
一项关于PWM刺激淋巴细胞中RNA和DNA合成动力学的研究表明，RNA合成比DNA合成开始早约24小时，且DNA合成和转化在66至78小时达到最大值。对PWM刺激培养物的组织化学和放射自显影研究表明，在72小时时，50%至60%的细胞群体已被PWM转化，并且出现了一种在细胞学上与早期浆细胞相似的独特细胞类型。PWM刺激细胞中新合成RNA的RNA沉降图谱显示，在24小时和40小时后形成了一个45至50S物质的大峰。因此，PWM可使人外周血淋巴细胞发生独特的转化。
