噬菌体MS2和T7在大肠杆菌链霉素抗性突变体上的生长情况。
Growth of bacteriophages MS2 and T7 on streptomycin-resistant mutants of Escherichia coli.
作者信息
Chakrabarti S, Gorini L
出版信息
J Bacteriol. 1975 Feb;121(2):670-4. doi: 10.1128/jb.121.2.670-674.1975.
Abstract
Streptomycin-resistant mutants of an Hfr strain of Escherichia coli K were examined for their ability to support the growth of male-specific ribonucleic acid phage MS2 and female-specific deoxyribonucleic acid phage T7. Normally, the Hfr strain allows propagation of MS2 and is lysed by it (efficiency of plating equal to 1), whereas the same strain restricts propagation of T7 and is not lysed by it (efficiency of plating smaller than 10-7). Twenty-four isolates out of 26 independently obtained streptomycin-resistant mutants are partially or completely derestricted for propagation of T7; efficiency of plating of T7 in such strains ranges from 10-3-1. Depending on their response to plating of MS2 and T7, the streptomycin-resistant mutants can be divided into four classes. The mutants in all four classes continue to be "male" in conjugation with F- strains. Genetic analysis is presented to show that restriction of MS2, derestriction of T7, and resistance to streptomycin are the pleiotropic effects of a single mutation at the strA locus.
摘要
对大肠杆菌K的一个Hfr菌株的链霉素抗性突变体进行了检测，以考察它们支持雄性特异性核糖核酸噬菌体MS2和雌性特异性脱氧核糖核酸噬菌体T7生长的能力。正常情况下，该Hfr菌株允许MS2繁殖并被其裂解（平板接种效率等于1），而同一菌株限制T7繁殖且不被其裂解（平板接种效率小于10-7）。在26个独立获得的链霉素抗性突变体中，有24个分离株对T7繁殖的限制被部分或完全解除；T7在此类菌株中的平板接种效率范围为10-3至1。根据它们对MS2和T7平板接种的反应，链霉素抗性突变体可分为四类。所有四类突变体在与F-菌株接合时仍为“雄性”。遗传分析表明，MS2的限制、T7的解除限制以及对链霉素的抗性是strA位点单一突变的多效性效应。
相似文献
1
Growth of bacteriophages MS2 and T7 on streptomycin-resistant mutants of Escherichia coli.噬菌体MS2和T7在大肠杆菌链霉素抗性突变体上的生长情况。
J Bacteriol. 1975 Feb;121(2):670-4. doi: 10.1128/jb.121.2.670-674.1975.
2
Male-specific bacteriophage MS2 propagation in fluorophenylalanine-resistant Escherichia coli K12.雄性特异性噬菌体MS2在抗氟苯丙氨酸的大肠杆菌K12中的增殖
J Virol. 1974 Jul;14(1):50-5. doi: 10.1128/JVI.14.1.50-55.1974.
3
Streptomycin-resistant Escherichia coli mutant temperature sensitive for the production of Qbeta-infective particles.对Qβ感染性颗粒产生具有温度敏感性的链霉素抗性大肠杆菌突变体。
J Virol. 1977 Jan;21(1):1-6. doi: 10.1128/JVI.21.1.1-6.1977.
4
Ampicillin-resistant mutants of Escherichia coli K-12 with lipopolysaccharide alterations affecting mating ability and susceptibility to sex-specific bacteriophages.大肠杆菌K-12的耐氨苄青霉素突变体，其脂多糖改变影响交配能力和对性别特异性噬菌体的敏感性。
J Bacteriol. 1971 Aug;107(2):420-32. doi: 10.1128/jb.107.2.420-432.1971.
5
Conditional lethal mutants of bacteriophage T4 unable to grow on a streptomycin resistant mutant of Escherichia coli.噬菌体T4的条件致死突变体无法在大肠杆菌的链霉素抗性突变体上生长。
Mutat Res. 1977 Aug;44(2):165-76. doi: 10.1016/0027-5107(77)90074-4.
6
Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7.无法支持T7在细胞内生长的大肠杆菌原养型突变体的分离与鉴定
J Virol. 1974 Sep;14(3):509-16. doi: 10.1128/JVI.14.3.509-516.1974.
7
Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage.Con突变体：大肠杆菌K-12中的一类突变体，缺乏一种主要的细胞壁蛋白，在噬菌体的接合和吸附方面存在缺陷。
J Bacteriol. 1974 Sep;119(3):726-35. doi: 10.1128/jb.119.3.726-735.1974.
8
F-Factor-mediated restriction of bacteriophage T7: synthesis of RNA and protein in T7-infected Escherichia coli F- and F+ cells.F因子介导的噬菌体T7限制作用：T7感染的大肠杆菌F-和F+细胞中RNA和蛋白质的合成
J Virol. 1975 Dec;16(6):1380-90. doi: 10.1128/JVI.16.6.1380-1390.1975.
9
Defective lysis of streptomycin-resistant escherichia coli cells infected with bacteriophage f2.用噬菌体f2感染的抗链霉素大肠杆菌细胞的裂解缺陷。
J Virol. 1981 Feb;37(2):813-20. doi: 10.1128/JVI.37.2.813-820.1981.
10
Revertants of double opal-mutants of bacteriophage T4.噬菌体T4双乳白突变体的回复突变体
Mol Gen Genet. 1976 Dec 22;149(3):357-8. doi: 10.1007/BF00268538.
引用本文的文献
1
Streptomycin- and rifampin-resistant mutants of Escherichia coli perturb F exclusion of bacteriophage T7 by affecting synthesis of the F plasmid protein PifA.大肠杆菌的链霉素和利福平抗性突变体通过影响F质粒蛋白PifA的合成来干扰噬菌体T7的F排斥作用。
J Bacteriol. 1995 Mar;177(6):1589-94. doi: 10.1128/jb.177.6.1589-1594.1995.
2
Leakage induced in Escherichia coli cells by A protein-RNA complexes from bacteriophage f2.噬菌体f2的A蛋白-RNA复合物在大肠杆菌细胞中诱导的渗漏。
J Virol. 1981 Jul;39(1):60-6. doi: 10.1128/JVI.39.1.60-66.1981.
3
Defective lysis of streptomycin-resistant escherichia coli cells infected with bacteriophage f2.用噬菌体f2感染的抗链霉素大肠杆菌细胞的裂解缺陷。
J Virol. 1981 Feb;37(2):813-20. doi: 10.1128/JVI.37.2.813-820.1981.
4
Inhibition of bacteriophage replication by extrachromosomal genetic elements.染色体外遗传元件对噬菌体复制的抑制作用。
Microbiol Rev. 1981 Mar;45(1):52-71. doi: 10.1128/mr.45.1.52-71.1981.
5
Recombinants between bacteriophages T7 and T3 which productively infect F-plasmid-containing strains of Escherichia coli.噬菌体T7和T3之间的重组体，可有效感染含有F质粒的大肠杆菌菌株。
J Virol. 1983 Jun;46(3):881-94. doi: 10.1128/JVI.46.3.881-894.1983.
6
Another gene affecting sexual expression of Escherichia coli.另一个影响大肠杆菌性表达的基因。
J Bacteriol. 1982 Apr;150(1):156-60. doi: 10.1128/jb.150.1.156-160.1982.
7
Errors and alternatives in reading the universal genetic code.通用遗传密码解读中的错误与变体
Microbiol Rev. 1989 Sep;53(3):273-98. doi: 10.1128/mr.53.3.273-298.1989.
8
Defective transcription of the right end of bacteriophage T7 DNA during an abortive infection of F plasmid-containing Escherichia coli.在含F质粒的大肠杆菌的流产感染过程中噬菌体T7 DNA右端的转录缺陷。
J Bacteriol. 1991 Feb;173(3):947-54. doi: 10.1128/jb.173.3.947-954.1991.
9
Genes 1.2 and 10 of bacteriophages T3 and T7 determine the permeability lesions observed in infected cells of Escherichia coli expressing the F plasmid gene pifA.噬菌体T3和T7的基因1.2和10决定了在表达F质粒基因pifA的大肠杆菌感染细胞中观察到的通透性损伤。
J Bacteriol. 1991 Oct;173(20):6507-14. doi: 10.1128/jb.173.20.6507-6514.1991.
10
Bacteriophage T7 morphogenesis and gene 10 frameshifting in Escherichia coli showing different degrees of ribosomal fidelity.噬菌体T7在大肠杆菌中的形态发生及基因10移码现象，该大肠杆菌表现出不同程度的核糖体保真度。
Mol Gen Genet. 1991 Dec;230(3):376-84. doi: 10.1007/BF00280294.
本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
3
Deficiency in initiation factors of protein synthesis induced by phage T7 in E. coli F(+) strains.噬菌体T7在大肠杆菌F(+)菌株中诱导的蛋白质合成起始因子缺乏。
FEBS Lett. 1972 Oct 15;27(1):1-4. doi: 10.1016/0014-5793(72)80394-6.
4
T7 translational control mechanisms and their inhibiton by F factors.T7 翻译控制机制及其受 F 因子的抑制作用。
Nat New Biol. 1971 May 12;231(19):37-41. doi: 10.1038/newbio231037a0.
5
Ribosomal discrimination of tRNAs.核糖体对转运RNA的识别
Nat New Biol. 1971 Dec 29;234(52):261-4. doi: 10.1038/newbio234261a0.
6
Genetic analysis of streptomycin resistance in Escherichia coli.大肠杆菌中链霉素抗性的遗传分析。
Genetics. 1970 May;65(1):9-25. doi: 10.1093/genetics/65.1.9.
7
A mutant of Escherichia coli with temperature-sensitive streptomycin protein.一种具有温度敏感性链霉素蛋白的大肠杆菌突变体。
Proc Natl Acad Sci U S A. 1970 Mar;65(3):544-50. doi: 10.1073/pnas.65.3.544.
8
Early stages of conjugation in Escherichia coli.大肠杆菌中接合作用的早期阶段。
J Bacteriol. 1969 Nov;100(2):1091-104. doi: 10.1128/jb.100.2.1091-1104.1969.
9
Extrachromosomal inheritance in bacteria.细菌中的染色体外遗传
Bacteriol Rev. 1969 Jun;33(2):210-63. doi: 10.1128/br.33.2.210-263.1969.
10
Role of 16S ribosomal ribonucleic acid and the 30S ribosomal protein S12 in the initiation of natural messenger ribonucleic acid translation.16S核糖体核糖核酸和30S核糖体蛋白S12在天然信使核糖核酸翻译起始中的作用
Biochemistry. 1974 May 7;13(10):2115-22. doi: 10.1021/bi00707a019.
Zotero 插件