Georgopoulos C P
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2977-81. doi: 10.1073/pnas.68.12.2977.
Bacterial mutants have been isolated, called groN, that block phage development by interference with the action of the product of the phage N gene. lambdatrp phages, which depend on the N product for the synthesis of tryptophan enzymes, do not make these enzymes in groN bacteria. Two type of phage mutants have been isolated that can overcome the groN block. One type makes an altered N product, the other contains an N-bypass mutation. The groN mutation is closely linked to the rifamycin-resistance locus in Escherichia coli. Purified RNA polymerase from the groN mutant is less activated by salt and more sensitive to rifamycin than is the polymerase from gro(+). This suggests that the groN mutation produces a structural change in the bacterial RNA polymerase such that it can no longer interact properly with the phage N product.
已经分离出了名为groN的细菌突变体，它们通过干扰噬菌体N基因产物的作用来阻断噬菌体的发育。依赖N产物合成色氨酸酶的λtrp噬菌体，在groN细菌中不能产生这些酶。已经分离出了两种能够克服groN阻断的噬菌体突变体。一种类型产生改变的N产物，另一种含有N-旁路突变。groN突变与大肠杆菌中的利福霉素抗性位点紧密连锁。与gro(+)菌株的聚合酶相比，来自groN突变体的纯化RNA聚合酶受盐的激活作用较小，对利福霉素更敏感。这表明groN突变在细菌RNA聚合酶中产生了结构变化，使其不能再与噬菌体N产物正常相互作用。
