萘啶酸对大肠杆菌核糖核酸合成的抑制作用。
Inhibition of ribonucleic acid synthesis by nalidixic acid in Escherichia coli.
作者信息
Javor G T
出版信息
J Bacteriol. 1974 Oct;120(1):282-6. doi: 10.1128/jb.120.1.282-286.1974.
Abstract
The effect of low concentrations of nalidixic acid on ribonucleic acid (RNA) synthesis in Escherichia coli was examined. It was observed that RNA synthesis in exponentially growing cells was not significantly affected, in harmony with previous studies. However, RNA synthesis was markedly depressed by nalidixic acid during starvation for an amino acid or during chloramphenicol treatment. This effect was not caused by increased killing or inhibition of nucleoside triphosphate synthesis by nalidixic acid. The pattern of radioactive uracil incorporation into transfer RNA or ribosomes was not changed by the drug. The sensitivity of RNA synthesis to nalidixic acid in the absence of protein production may be useful in probing the amino acid control of RNA synthesis.
摘要
研究了低浓度萘啶酸对大肠杆菌核糖核酸（RNA）合成的影响。观察到，与先前的研究一致，处于指数生长期的细胞中的RNA合成未受到显著影响。然而，在氨基酸饥饿期间或氯霉素处理期间，萘啶酸会显著抑制RNA合成。这种效应并非由萘啶酸导致的细胞杀伤增加或对核苷三磷酸合成的抑制引起。该药物并未改变放射性尿嘧啶掺入转运RNA或核糖体的模式。在不进行蛋白质合成的情况下，RNA合成对萘啶酸的敏感性可能有助于探究RNA合成的氨基酸调控机制。
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