Miller G G, Witwer M W, Braude A I, Davis C E
J Clin Invest. 1974 Nov;54(5):1235-40. doi: 10.1172/JCI107867.
Gas-liquid chromatography (GLC) was used to study normal serum and serum from patients with septicemia caused by a variety of bacteria and by Candida albicans. The gas chromatograms of seven sera from six patients with septicemia due to C. albicans were found to be significantly and reproducibly different from those of normal sera. Chromatograms of serum from 19 bacteremic patients were indistinguishable from normals. The major peaks present in chromatograms of normal sera were identified by GLC and mass spectroscopy as the methyl esters of palmitic, oleic, linoleic, and stearic acids. In addition to these peaks, serum from patients with candidemia contained abnormal peaks that were also present in cultures of C. albicans grown in normal serum and in washed C. albicans harvested from cultures in yeast nitrogen base broth. Chromatograms from 11 cases of mucosal candidates differed little from normal and were easily distinguished from those of fungemia patients. Chromatograms of serum from two of four patients with deep-invasive candidiasis were indistinguishable from those of fungemia and reverted to normal after infections were eradicated.
采用气液色谱法（GLC）对正常血清以及由多种细菌和白色念珠菌引起败血症患者的血清进行了研究。发现6例白色念珠菌败血症患者的7份血清的气相色谱图与正常血清的气相色谱图存在显著且可重复的差异。19例菌血症患者血清的色谱图与正常血清无法区分。通过气液色谱法和质谱法鉴定出正常血清色谱图中的主要峰为棕榈酸、油酸、亚油酸和硬脂酸的甲酯。除了这些峰外，念珠菌血症患者的血清中还含有异常峰，这些峰也存在于在正常血清中生长的白色念珠菌培养物以及从酵母氮基肉汤培养物中收获的洗涤后的白色念珠菌中。11例黏膜念珠菌病患者的色谱图与正常血清差异不大，且很容易与真菌血症患者的色谱图区分开来。4例深部侵袭性念珠菌病患者中有2例血清的色谱图与真菌血症患者的无法区分，感染根除后恢复正常。
