膜磷脂合成存在缺陷的大肠杆菌突变体：L-甘油-3-磷酸脱氢酶结构基因的定位
Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase.
作者信息
Cronan J E, Bell R M
出版信息
J Bacteriol. 1974 May;118(2):598-605. doi: 10.1128/jb.118.2.598-605.1974.
Abstract
The structural gene for the biosynthetic l-glycerol 3-phosphate dehydrogenase has been mapped at min 71.5 on the Escherichia coli chromosome. This gene (gpsA) is co-transduced with the xyl, mtl, and pyrE loci. Three-factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosone is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature-sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase-deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.
摘要
生物合成L-甘油-3-磷酸脱氢酶的结构基因已定位在大肠杆菌染色体的71.5分钟处。该基因（gpsA）与木糖、甘露糖和pyrE位点共转导。三因子结合杂交和转导数据表明，染色体该区域的位点顺序为甘露糖、gltE、gpsA、gadR、gadS、pyrE。对具有热稳定性增加的脱氢酶的温度敏感gpsA突变体的研究表明，gpsA是该脱氢酶的结构基因。所有测试的脱氢酶缺陷菌株都定位在非常靠近gpsA位点的位置。遗传互补分析的尝试未成功。
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