Baer H, Washington L
Appl Microbiol. 1972 Jan;23(1):108-12. doi: 10.1128/am.23.1.108-112.1972.
A numerical diagnostic key for enteric organisms is described which permits the identification of typical strains and of biochemical variants with high accuracy. Unknown strains are inoculated into a basic set of five media which permit the testing of eight biochemical reactions. The positive reactions are assigned points, and the score of a strain is added up, after which the identification of the strain is obtained from a table. In many instances, the final identification is obtained with this set of biochemical tests; and, in other instances, a small number of additional tests are required to distinguish between organisms giving the same score in the basic set of biochemical tests. The key permits an accurate, rapid, and economical differentiation of the typical and the more common atypical biotypes of enteric organisms in the clinical laboratory.
本文描述了一种用于肠道微生物的数字诊断密钥，它能够高精度地鉴定典型菌株和生化变体。将未知菌株接种到一组基本的五种培养基中，这组培养基可用于检测八种生化反应。对阳性反应进行计分，并将菌株的分数相加，然后从表格中得出该菌株的鉴定结果。在许多情况下，通过这组生化试验就能得到最终鉴定结果；而在其他情况下，则需要进行少量额外试验，以区分在基本生化试验组中得分相同的微生物。该密钥能够在临床实验室中准确、快速且经济地鉴别肠道微生物的典型和较常见的非典型生物型。
