Ashdown L R
Pathology. 1979 Apr;11(2):293-7. doi: 10.3109/00313027909061954.
A selective medium consisting of trypticase soy agar with 4% glycerol, 5 mg/l crystal violet, 50 mg/l neutral red and 4 mg/l of gentamicin was devised for isolation of Pseudomonas pseudomallei from clinical specimens. Absorption of neutral red was found to be suitable for differentiating this organism from other bacteria, while gentamicin was effective in selecting Ps. pseudomallei from organisms commonly found in clinical material. The medium was more suitable for screening clinical specimens than MacConkey's agar with colistin-S because it was more selective and allowed multiple specimens to be inoculated on a single plate. Eight thousand clinical specimens from an area endemic for melioidosis were screened on the selective medium. This resulted in the recovery of 8 isolates of Ps. pseudomallei that would not have been detected using routine culture media alone.
设计了一种选择性培养基，其由含有4%甘油、5mg/L结晶紫、50mg/L中性红和4mg/L庆大霉素的胰蛋白胨大豆琼脂组成，用于从临床标本中分离类鼻疽假单胞菌。发现中性红的吸收适合于将该菌与其他细菌区分开来，而庆大霉素能有效地从临床材料中常见的微生物中筛选出类鼻疽假单胞菌。该培养基比含多黏菌素-S的麦康凯琼脂更适合筛选临床标本，因为它具有更高的选择性，并且允许在单个平板上接种多个标本。在该选择性培养基上对来自类鼻疽病流行地区的8000份临床标本进行了筛选。这使得分离出了8株类鼻疽假单胞菌，而仅使用常规培养基这些菌株是无法被检测到的。
