大规模制备共价闭合λDNA分子的新方法。
New method for large-scale preparation of covalently closed lambda DNA molecules.
作者信息
Reuben R, Gefter M, Enquist L, Skalka A
出版信息
J Virol. 1974 Nov;14(5):1104-7. doi: 10.1128/JVI.14.5.1104-1107.1974.
Abstract
A combination of mutations in bacteriophage lambda and its host Escherichia coli K-12 provides a convenient system for the isolation of large quantities of covalently closed circular DNA molecules. We describe two procedures for the large scale preparation of lambda DNA in the duplex circular form.
摘要
噬菌体λ及其宿主大肠杆菌K-12中的一系列突变组合，为大量分离共价闭合环状DNA分子提供了一个便利的系统。我们描述了两种大规模制备双链环状形式λDNA的方法。
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