核酸外切酶VIII的分离：与sbcA间接抑制因子相关的酶
Isolation of exonuclease VIII: the enzyme associated with sbcA indirect suppressor.
作者信息
Kushner S R, Nagaishi H, Clark A J
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3593-7. doi: 10.1073/pnas.71.9.3593.
Abstract
recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed by the presence of sbcA(-) mutations. sbcA(-) strains contain an increased level of an ATP-independent nuclease. Genetic and enzymatic tests indicate that this activity is not exonuclease III, exonuclease V (recB-recC nuclease), DNA polymerase I, or lambda exonuclease. This new enzyme (exonuclease VIII) has been purified 750-fold and shows a striking preference for double-stranded DNA over heat-denatured DNA. It does not act endonucleolytically on closed circular, single-stranded DNA as exonuclease V does. It also lacks a 3'-phosphatase function. Analysis on sodium dodecyl sulfate-polyacrylamide gels indicates that exonuclease VIII is not present in unsuppressed (sbcA(+)) strains. It is thought that sbcA determines some type of control function; the structural gene for exonuclease VIII is denoted by recE.
摘要
大肠杆菌K - 12中recB和/或recC缺陷可被sbcA(-)突变的存在间接抑制。sbcA(-)菌株中一种不依赖ATP的核酸酶水平升高。遗传和酶学测试表明，这种活性不是核酸外切酶III、核酸外切酶V（recB-recC核酸酶）、DNA聚合酶I或λ核酸外切酶。这种新酶（核酸外切酶VIII）已被纯化750倍，并且与热变性DNA相比，对双链DNA表现出明显的偏好。它不像核酸外切酶V那样对闭环单链DNA进行内切核酸酶作用。它也缺乏3'-磷酸酶功能。十二烷基硫酸钠-聚丙烯酰胺凝胶分析表明，核酸外切酶VIII不存在于未被抑制的（sbcA(+)）菌株中。据认为，sbcA决定某种类型的控制功能；核酸外切酶VIII的结构基因由recE表示。
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