Miyada C G, Horwitz A H, Cass L G, Timko J, Wilcox G
Nucleic Acids Res. 1980 Nov 25;8(22):5267-74. doi: 10.1093/nar/8.22.5267.
The DNA sequence of the araC regulatory gene from Escherichia coli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation in araC, delta(araCO)719, removes all but the last 22 codons of the gene.
利用马克萨姆和吉尔伯特的碱基特异性化学切割反应，已测定了来自大肠杆菌B/r的araC调控基因的DNA序列。发现一个开放阅读框，其编码一个由292个氨基酸组成的蛋白质。一个无义突变araC5被证明是由核苷酸429处的G到A的转换导致的，该转换将色氨酸密码子TGG转变为琥珀密码子TAG。一个不与araC中任何已知点突变重组的缺失突变体delta(araCO)719，去除了该基因除最后22个密码子之外的所有部分。
