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鼠伤寒沙门氏菌LT2阿拉伯糖突变的遗传学特征分析

Genetic characterization of Salmonella typhimurium LT2 ara mutations.

作者信息

Lee J H, Nishitani J, Wilcox G

出版信息

J Bacteriol. 1984 Apr;158(1):344-6. doi: 10.1128/jb.158.1.344-346.1984.

Abstract

Seventeen independently isolated L-arabinose utilization-deficient mutants of Salmonella typhimurium LT2 were characterized. Four complementation groups (araA, araB, araC, and araD) were identified and were equivalent to the same genes in the ara system in Escherichia coli. The order of the four genes was determined to be araD-araA-araB-araC-leu. Two transcription units were found: the araBAD operon was transcribed counterclockwise, and the araC gene was transcribed clockwise.

摘要

对17个独立分离得到的鼠伤寒沙门氏菌LT2的L-阿拉伯糖利用缺陷型突变体进行了表征。鉴定出四个互补群(araA、araB、araC和araD),它们与大肠杆菌ara系统中的相同基因相对应。确定这四个基因的顺序为araD-araA-araB-araC-leu。发现了两个转录单元:araBAD操纵子逆时针转录,araC基因顺时针转录。

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