多核苷酸。L.聚（2'-氯-2'-脱氧腺苷酸）和聚（2'-溴-2'-脱氧腺苷酸）的合成及性质
Polynucleotides. L. Synthesis and properties of poly (2'-chloro-2'-deoxyadenylic acid) and poly (2'-bromo-2'-deoxyadenylic acid).
作者信息
Ikehara M, Fukui T, Kakiuchi N
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4249-60. doi: 10.1093/nar/4.12.4249.
Abstract
Poly (2'-chloro-2'-deoxyadenylic acid) and poly (2'-bromo-2'-deoxyadenylic acid) were synthesized from the corresponding diphosphates with the aid of polynucleotide phosphorylase from E. coli. UV, CD, acid titration and mixing with poly (U) were investigated. Comparing these properties with those of poly (A) and poly (2'-azido-2'-deoxyadenylic acid), it was found that 2''substituents exert significant effects on the thermal stability of these polynucleotides, though the overall conformational structure was not greatly changed.
摘要
聚（2'-氯-2'-脱氧腺苷酸）和聚（2'-溴-2'-脱氧腺苷酸）由相应的二磷酸酯借助大肠杆菌的多核苷酸磷酸化酶合成。对其进行了紫外、圆二色、酸滴定以及与聚（U）混合等研究。将这些性质与聚（A）和聚（2'-叠氮基-2'-脱氧腺苷酸）的性质进行比较后发现，尽管整体构象结构变化不大，但2''取代基对这些多核苷酸的热稳定性有显著影响。
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