Tate V E, Philipson L
Nucleic Acids Res. 1979 Jun 25;6(8):2769-85. doi: 10.1093/nar/6.8.2769.
Micrococcal-nuclease digestion of adenovirus 2(ad 2) infected HeLa cell nuclei early after infection has been used to investigate the nucleoprotein nature of parental viral DNA. Viral DNA is more susceptible to nuclease digestion than cellular DNA. The pattern of digestion products changes as digestion proceeds from an indistinct pattern 1 hour post infection(pi) to a nucleosome-like pattern at 6 hours pi. The major differences between viral and cellular nucleoprotein products were i) a subnucleosome fraction from viral DNA and ii) the repeat size of DNA in viral nucleosomes was 165 base pairs and in cellular nucleosomes, 195 base pairs. Up to 50% viral DNA in nuclei 6 hours pi seems to be in nucleosome-like structures. Such patterns are not seen on digestion of partially-uncoated virus or isolated cores.
在感染腺病毒2（Ad2）的HeLa细胞核感染后早期，用微球菌核酸酶消化来研究亲代病毒DNA的核蛋白性质。病毒DNA比细胞DNA更易受核酸酶消化。随着消化从感染后1小时（pi）的不清晰模式发展到感染后6小时的核小体样模式，消化产物的模式发生变化。病毒和细胞核蛋白产物之间的主要差异在于：i）病毒DNA的亚核小体部分；ii）病毒核小体中DNA的重复长度为165个碱基对，而细胞核小体中为195个碱基对。感染后6小时，细胞核中高达50%的病毒DNA似乎处于核小体样结构中。在部分未包被的病毒或分离的核心进行消化时，看不到这种模式。
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