来自支原体（Kid）的苯丙氨酸转运RNA：一种缺乏在蛋白质合成中起作用的超修饰核苷的转运RNA。
The phenylalanine tRNA from Mycoplasma sp. (Kid): a tRNA lacking hypermodified nucleosides functional in protein synthesis.
作者信息
Kimball M E, Soll D
出版信息
Nucleic Acids Res. 1974 Dec;1(12):1713-20.
Abstract
Phenylalanine tRNA from Mycoplasma sp. (Kid) was purified and characterized. The tRNA can be aminoacylated by phenylalanyl-tRNA synthetase from both Mycoplasma and E. coli. In a tRNA-dependent cell-free E. coli amino acid incorporating system programmed with poly U pure Mycoplasma tRNA(Phe) was fully active in promoting phenylalanine incorporation, even in direct competition with homologous E. coli tRNA(Phe). Since the Mycoplasma tRNA lacks isopentenyladenosine, or any related hypermodified nucleoside, it appears that the presence of such nucleosides in tRNA is not an absolute requirement for protein synthesis.
摘要
对来自支原体属（Kid株）的苯丙氨酸转运核糖核酸（tRNA）进行了纯化和特性分析。该tRNA可被来自支原体和大肠杆菌的苯丙氨酰-tRNA合成酶氨酰化。在以聚尿苷酸编程的依赖于tRNA的无细胞大肠杆菌氨基酸掺入系统中，纯的支原体tRNA（Phe）在促进苯丙氨酸掺入方面完全具有活性，即使在与同源的大肠杆菌tRNA（Phe）直接竞争时也是如此。由于支原体tRNA缺乏异戊烯基腺苷或任何相关的超修饰核苷，因此似乎tRNA中此类核苷的存在并非蛋白质合成的绝对必要条件。
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The phenylalanine tRNA from Mycoplasma sp. (Kid): a tRNA lacking hypermodified nucleosides functional in protein synthesis.来自支原体（Kid）的苯丙氨酸转运RNA：一种缺乏在蛋白质合成中起作用的超修饰核苷的转运RNA。
Nucleic Acids Res. 1974 Dec;1(12):1713-20.
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引用本文的文献
1
Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase.借助多核苷酸磷酸化酶和多核苷酸激酶对非放射性支原体苯丙氨酸tRNA进行序列研究。
Nucleic Acids Res. 1974 Dec;1(12):1733-8. doi: 10.1093/nar/1.12.1733.
2
The nucleotide sequence of phenylalanine tRNA from Mycoplasma sp. (Kid).来自支原体（Kid株）的苯丙氨酸tRNA的核苷酸序列。
Nucleic Acids Res. 1974 Dec;1(12):1721-32.
3
Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae.来自酿酒酵母反抑制突变体的异戊烯基腺苷缺陷型tRNA。
Nucleic Acids Res. 1978 Nov;5(11):4329-42. doi: 10.1093/nar/5.11.4329.
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The mycoplasmas.支原体。
Microbiol Rev. 1978 Jun;42(2):414-70. doi: 10.1128/mr.42.2.414-470.1978.
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Chemical modification of tRNA-Tyr-yeast with bisulfite. A new method to modify isopentenyladenosine residue.用亚硫酸氢盐对酵母tRNA-Tyr进行化学修饰。一种修饰异戊烯基腺苷残基的新方法。
Biochem Biophys Res Commun. 1970 Dec 9;41(5):1185-91. doi: 10.1016/0006-291x(70)90211-1.
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[Modification of serine-specific transfer ribonucleic acid by iodine].[丝氨酸特异性转移核糖核酸的碘修饰]
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Transfer ribonucleic acid deficient in N6-(delta 2-isopentenyl)adenosine due to mevalonic acid limitation.由于甲羟戊酸限制而缺乏N6-（δ2-异戊烯基）腺苷的转移核糖核酸
Biochemistry. 1971 Mar 16;10(6):994-1001. doi: 10.1021/bi00782a010.
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Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.酪氨酸转运核糖核酸中的碱基修饰：一种影响核糖体结合的修饰碱基。
J Mol Biol. 1969 Jan 14;39(1):145-57. doi: 10.1016/0022-2836(69)90339-8.
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Studies on polynucleotides. LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression.多核苷酸研究。第八十五部分。琥珀抑制tRNA的部分纯化及体外抑制研究。
J Mol Biol. 1968 May 28;34(1):175-87. doi: 10.1016/0022-2836(68)90243-x.
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N6-(delta 2-isopentenyl)adenosine: chemical reactions, biosynthesis, metabolism, and significance to the structure and function of tRNA.N6-（δ2-异戊烯基）腺苷：化学反应、生物合成、代谢及其对转运核糖核酸结构和功能的意义
Prog Nucleic Acid Res Mol Biol. 1970;10:57-86. doi: 10.1016/s0079-6603(08)60561-9.
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