来自酿酒酵母反抑制突变体的异戊烯基腺苷缺陷型tRNA。
Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae.
作者信息
Laten H, Gorman J, Bock R M
出版信息
Nucleic Acids Res. 1978 Nov;5(11):4329-42. doi: 10.1093/nar/5.11.4329.
Abstract
We have isolated a mutant of Saccharomyces cerevisiae that contains 1.5% of the normal tRNA complement of isopentenyladenosine (i6A). The mutant was characterized by the reduction in efficiency of a tyrosine inserting UAA nonsense suppressor. The chromatographic profiles of tRNATyr and tRNASer on benzoylated DEAE-cellulose are consistent with the loss of i6A by these species. Transfer RNA from the mutant exhibits 6.5% of the cytokinin biological activity expected for yeast tRNA. Transfer RNAs from the mutant that normally contain i6A accept the same levels of amino acids in vitro as the fully modified species. With the exception of i6A, the level of modified bases in unfractionated tRNA from the mutant appears to be normal. The loss of i6A apparently affects tRNA's role in protein synthesis at a step subsequent to aminoacylation.
摘要
我们分离出了一株酿酒酵母突变体，其异戊烯基腺苷（i6A）的tRNA含量仅为正常水平的1.5%。该突变体的特征是酪氨酸插入UAA无义抑制子的效率降低。tRNATyr和tRNASer在苯甲酰化DEAE - 纤维素上的色谱图谱与这些种类中i6A的缺失一致。来自突变体的转移RNA表现出酵母tRNA预期的细胞分裂素生物活性的6.5%。来自突变体的通常含有i6A的转移RNA在体外接受氨基酸的水平与完全修饰的种类相同。除了i6A之外，来自突变体的未分级tRNA中修饰碱基的水平似乎是正常的。i6A的缺失显然在氨酰化之后的步骤影响了tRNA在蛋白质合成中的作用。
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