Helmke K, Federlin K, Piazolo P, Ströder J, Jeschke R, Franz H E
Gut. 1974 Nov;15(11):875-9. doi: 10.1136/gut.15.11.875.
Antiserum directed against calcium-binding protein isolated from human kidneys was used for the immunofluorescent localization of calcium-binding protein in human intestine and kidney. Frozen sections of intestine obtained by biopsy from normal persons were tested by the indirect fluorescent antibody technique. Specific fluorescence indicating the presence of calciumbinding protein was observed at both the basal and apical poles of the intestinal absorptive cells while the goblet cells appeared to fluoresce non-specifically. Treatment of rachitic children with 25-hydroxycholecalciferol generally restored the pattern of fluorescence seen in intestinal tissue from normal persons. Examinations of intestinal biopsies from uraemic patients yielded variable results.
针对从人肾脏分离出的钙结合蛋白的抗血清，用于钙结合蛋白在人肠道和肾脏中的免疫荧光定位。通过活检从正常人获得的肠道冰冻切片，采用间接荧光抗体技术进行检测。在肠道吸收细胞的基极和顶端均观察到指示钙结合蛋白存在的特异性荧光，而杯状细胞似乎呈现非特异性荧光。用25-羟胆钙化醇治疗佝偻病儿童，通常可使肠道组织恢复到正常人所见的荧光模式。对尿毒症患者肠道活检的检查结果各异。
