青霉素、头孢菌素和青霉素酶的自动化测定方法。
Automated method for determination of penicillins, cephalosporins, and penicillinases.
作者信息
Lindström E B, Nordström K
出版信息
Antimicrob Agents Chemother. 1972 Feb;1(2):100-6. doi: 10.1128/AAC.1.2.100.
Abstract
The microiodometric assay of Novick for penicillinase was adapted to the Technicon Autoanalyzer system. With this automated method, it is possible to analyze almost 60 samples per hr. Two different procedures are described: in the first, the samples were incubated with substrate in the machine under standardized conditions; in the second, samples were incubated outside the Autoanalyzer and assayed at intervals for penicilloic acid. As little as 5 mum penicilloic acid could easily be detected in a sample of 0.4 ml. The reproducibility was very good. The hydrolysis of penicillin in a suspension of nongrowing bacteria as well as in a growing culture could also be followed. The automated method has been used both for kinetic studies and in screening for activity in eluates from column chromatography experiments.
摘要
诺维克青霉素酶微量碘量测定法被应用于Technicon自动分析仪系统。采用这种自动化方法，每小时可分析近60个样品。文中描述了两种不同的程序：第一种是将样品在机器中与底物在标准化条件下孵育；第二种是将样品在自动分析仪外部孵育，并定期检测青霉素酸。在0.4毫升的样品中，很容易检测到低至5微克的青霉素酸。重现性非常好。还可以跟踪青霉素在非生长细菌悬浮液以及生长培养物中的水解情况。这种自动化方法已用于动力学研究以及柱色谱实验洗脱液活性的筛选。
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本文引用的文献
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Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants.大肠杆菌对青霉素的耐药性。I. 一些氨苄青霉素耐药突变体的遗传学研究。
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Micro-iodometric assay for penicillinase.青霉素酶的微量碘量法测定
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A DIRECT SPECTROPHOTOMETRIC ASSAY FOR PENICILLIN BETA-LACTAMASE (PENICILLINASE).一种用于青霉素β-内酰胺酶（青霉素酶）的直接分光光度测定法。
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The estimation of penicillins and penicillin destruction.青霉素及其降解产物的测定
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Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
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Iodometric assay of penicillinase.青霉素酶的碘量法测定
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Resistance of Escherichia coli to penicillins. V. Physiological comparison of two isogenic strains, one with chromosomally and one with episomally mediated ampicillin resistance.大肠杆菌对青霉素的耐药性。V. 两个同基因菌株的生理学比较，一个具有染色体介导的氨苄青霉素耐药性，另一个具有附加体介导的氨苄青霉素耐药性。
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