Corbeil M, Trudel M, Payment P
J Clin Microbiol. 1979 Jul;10(1):91-5. doi: 10.1128/jcm.10.1.91-95.1979.
A novel method for large-scale culture of anchorage-dependent cells is described. Culture vessels were constructed by using roller bottles and Pyrex tubing. Parallel glass tubes, equally spaced by silicone rings and inserted into the roller bottles, provided an increased culture area. The roller bottles were fitted with a second opening for medium recirculation. Culture vessels of 1,000-, 4,000-, 8,000- and 16,000-cm2 growth area were constructed to propagate Vero, BS-C-1, primary chicken embryo, and IMH-P cells. These cultured cells were used to efficiently produce several viruses: rubella virus, measles virus, poliovirus, cytomegalovirus, and herpesvirus of turkey.
描述了一种用于贴壁依赖性细胞大规模培养的新方法。培养容器通过使用滚瓶和派热克斯玻璃管构建而成。由硅树脂环等距隔开并插入滚瓶中的平行玻璃管提供了更大的培养面积。滚瓶配有第二个开口用于培养基再循环。构建了生长面积为1000、4000、8000和16000平方厘米的培养容器，用于培养非洲绿猴肾细胞、非洲绿猴肾传代细胞、原代鸡胚细胞和IMH-P细胞。这些培养的细胞被用于高效生产几种病毒：风疹病毒、麻疹病毒、脊髓灰质炎病毒、巨细胞病毒和火鸡疱疹病毒。
