两种蛋白质因子对大肠杆菌提取物中RNA合成的刺激作用。
Stimulation of RNA synthesis by two protein factors in extracts of Escherichia coli.
作者信息
Murooka Y, Lazzarini R A
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2336-40. doi: 10.1073/pnas.69.8.2336.
Abstract
Two separate protein factors that markedly stimulate DNA-dependent RNA polymerase have been partially purified from ribosome-free extracts of E. coli Q13 cells. These proteins appear to differ from sigma, M, H, psi, and the factor described by Mahadik and Srinivasan. The stimulation by either factor requires the presence of the sigma protein. Using RNA-DNA hybridization competition analyses, we also demonstrate that neither of the new factors preferentially stimulates the synthesis of ribosomal RNA. The purification, properties, and mechanism of action of these new factors are examined.
摘要
从大肠杆菌Q13细胞的无核糖体提取物中已部分纯化出两种能显著刺激依赖DNA的RNA聚合酶的不同蛋白质因子。这些蛋白质似乎不同于σ因子、M因子、H因子、ψ因子以及马哈迪克和斯里尼瓦桑所描述的因子。任一因子的刺激作用都需要σ蛋白的存在。通过RNA-DNA杂交竞争分析，我们还证明这两种新因子均不会优先刺激核糖体RNA的合成。我们对这些新因子的纯化、特性及作用机制进行了研究。
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