Dhir S P, Kenny G E, Grayston J T
Infect Immun. 1971 Dec;4(6):725-30. doi: 10.1128/iai.4.6.725-730.1971.
A lipid complement-fixing group antigen is shared by all chlamydiae including trachoma organisms. A water-soluble polysaccharide antigen was obtained by alkali saponification from partially purified water-insoluble lipid antigen preparations made from three trachoma strains. The extracted antigen failed to fix complement with antibody but was capable, as a hapten, of inhibiting complement fixation by prior reaction with the antibody. This antigen diffused readily in agarose, producing a reaction of identity with several trachoma strains. The antigen could also be absorbed onto untreated rabbit red blood cells for passive hemagglutination. It is concluded that polysaccharide antigen is responsible for the group reactivity of these organisms and can be obtained from the lipid antigen by alkali saponification.
包括沙眼病原体在内的所有衣原体都共享一种脂质补体结合组抗原。通过碱皂化法从由三株沙眼菌株制备的部分纯化的水不溶性脂质抗原制剂中获得了一种水溶性多糖抗原。提取的抗原不能与抗体结合补体，但作为半抗原，通过与抗体预先反应能够抑制补体结合。这种抗原在琼脂糖中易于扩散，与几种沙眼菌株产生同一性反应。该抗原也可以吸附到未处理的兔红细胞上用于被动血凝试验。得出的结论是，多糖抗原是这些生物体群体反应性的原因，并且可以通过碱皂化从脂质抗原中获得。
