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淋病奈瑟菌感染的研究。II. 淋病奈瑟菌的冷冻断裂、冷冻蚀刻研究。

Studies on gonococcus infection. II. Freeze-fracture, freeze-etch studies on gonocci.

作者信息

Swanson J

出版信息

J Exp Med. 1972 Nov 1;136(5):1258-71. doi: 10.1084/jem.136.5.1258.

Abstract

Gonococci have been studied by electron microscopy after freeze-cleavage, freeze-etching and the findings correlated with those obtainable through thin sectioning and negative staining. The outer membrane of the cell wall is composed of round to hexagonal subunits 80 A in diameter. This membrane is also punctuated by 80-A holes visible on the exterior of the organism and extending into the substance or through the outer membrane. Pili coursing over the surface of the organisms appear to maintain a close anatomical relationship with the cell wall. In some instances, the surfaces of the organisms are virtually covered by a layer of pili.

摘要

通过冷冻断裂、冷冻蚀刻电子显微镜对淋球菌进行了研究,并将研究结果与通过超薄切片和负染色获得的结果进行了对比。细胞壁的外膜由直径80埃的圆形至六边形亚基组成。该膜上还有一些80埃的小孔,在菌体表面可见,这些小孔向内延伸至菌体内或穿过外膜。菌体表 面的菌毛似乎与细胞壁保持着紧密的解剖学关系。在某些情况下,菌体表面实际上被一层菌毛覆盖。

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