Prouvost-Danon A, Binaghi R, Rochas S, Boussac-Aron Y
Immunology. 1972 Oct;23(4):481-91.
Immunochemical identification of a distinct immunoglobulin class associated with mouse reaginic antibody and designated IgE has been performed. An antiserum against mouse reaginic antibody was prepared in rats by immunization with homologous peritoneal mast cells sensitized with mouse reaginic antibody. This antiserum, after adequate absorption, recognized only one component existing in mouse serum or fractions of serum containing reaginic antibody. This component was not any of the already known γ, γ, IgA or IgM immunoglobulins; its immunoglobulin nature was indicated by its antigen-binding capacity in radioimmunodiffusion analysis. Fractionation experiments (zone electrophoresis, gel filtration on Sephadex G-200) showed that there was a strict association between reaginic anaphylactic activity and IgE immunoglobulin. Molecular weight of IgE was found to be about 200,000. In biological tests, anti-IgE neutralized anaphylactic activity attributed to reaginic antibody, but not that attributed to γ-antibody. Anti-IgE degranulates normal and sensitized mouse peritoneal mast cells, and rat peritoneal mast cells after they have been sensitized by mouse reaginic antibody.
已对与小鼠反应素抗体相关的一种独特免疫球蛋白类别进行了免疫化学鉴定，并将其命名为IgE。通过用小鼠反应素抗体致敏的同源腹膜肥大细胞免疫大鼠，制备了抗小鼠反应素抗体的抗血清。该抗血清在充分吸收后，仅识别存在于小鼠血清或含有反应素抗体的血清组分中的一种成分。该成分不是任何一种已知的γ、γ、IgA或IgM免疫球蛋白；其免疫球蛋白性质通过放射免疫扩散分析中的抗原结合能力得以表明。分级分离实验（区带电泳、在Sephadex G - 200上进行凝胶过滤）表明，反应素过敏活性与IgE免疫球蛋白之间存在严格关联。发现IgE的分子量约为200,000。在生物学试验中，抗IgE中和了归因于反应素抗体的过敏活性，但未中和归因于γ抗体的过敏活性。抗IgE使正常和致敏的小鼠腹膜肥大细胞以及经小鼠反应素抗体致敏后的大鼠腹膜肥大细胞脱颗粒。
