Vi抗原的分离及其简单测定方法。
Isolation of Vi antigen and a simple method for its measurement.
作者信息
Wong K H, Feeley J C
出版信息
Appl Microbiol. 1972 Oct;24(4):628-33. doi: 10.1128/am.24.4.628-633.1972.
Abstract
Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.
摘要
Vi抗原是从柠檬酸杆菌5396/38菌株的盐水提取物中通过酶消化、乙醇浓缩和十六烷基三甲基溴化铵沉淀而纯化得到的。用这种方法制备的Vi抗原在小鼠体内的保护作用比通过早期利用酸水解方法分离得到的另外两种制剂分别高18倍和230倍。描述了一种用于测定Vi抗原的灵敏且特异的电免疫扩散方法。
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